PMID: 11933070Apr 5, 2002Paper

Comparative structural analysis of psychrophilic and meso- and thermophilic enzymes

Proteins
G GianeseStefano Pascarella

Abstract

Enzymes adapted to cold display structures comparable with those of their meso- and thermophilic homologs but are characterized by a higher catalytic efficiency at low temperatures and by thermolability at moderate temperatures. To identify the structural factors responsible of such features, we undertook a systematic comparative analysis of several structural properties in a data set consisting of 7 cold active enzymes belonging to different structural families and 28 related structures from meso/thermophiles representing most of the structural information now available. Only high-resolution and high-quality structures were considered. Properties were calculated and then compared for each pair of 3D structures displaying different temperatures of adaptation using a temperature-weighting scheme. The significance of the resulting differences was evaluated with a statistical method. Results reveal that each protein family adopts different structural strategies to adapt to low temperatures. However, some common trends are observed: the number of ion pairs, the side-chain contribution to the exposed surface, and the apolar fraction of the buried surface show a consistent decrease with decreasing optimal temperatures.

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·F M Richards
Aug 7, 1990·Biochemistry·K A Dill
Nov 16, 1989·Nature·L Serrano, A R Fersht
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jun 17, 1986·Biochemistry·A A RashinB Honig
Jan 1, 1984·Progress in Biophysics and Molecular Biology·E N Baker, R E Hubbard
Aug 25, 1983·Journal of Molecular Biology·D J Barlow, J M Thornton
Apr 25, 1980·Journal of Molecular Biology·M R MurthyM G Rossmann
Dec 8, 1995·Journal of Molecular Biology·B MusafiaD Arad
May 1, 1994·Protein Engineering·S J HubbardP Argos
May 20, 1994·Journal of Molecular Biology·I K McDonald, J M Thornton
Mar 20, 1993·Journal of Molecular Biology·K IshikawaK Morikawa
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N PaceK Gajiwala
Dec 1, 1995·Protein Science : a Publication of the Protein Society·A H JufferP Argos
May 23, 1996·Nature·R W HooftE E Abola
Mar 1, 1996·Protein Engineering·E QuerolA Mozo-Villarias
Dec 15, 1995·Structure·A Goldman
Dec 1, 1995·Proteins·D Frishman, P Argos
Oct 1, 1996·Biophysical Journal·J K Myers, C N Pace
Nov 1, 1995·Journal of Molecular Recognition : JMR·N PattabiramanP J Fleming
Apr 1, 1996·Proteins·L Zhang, J Hermans
Jun 20, 1997·Journal of Molecular Biology·G VogtP Argos
Jan 1, 1997·Folding & Design·G Vogt, P Argos
Sep 18, 1997·Trends in Biotechnology·C J Marshall
Aug 1, 1997·Journal of Biochemistry·G GrazianoG Barone
Feb 12, 1998·Protein Science : a Publication of the Protein Society·V VilleretJ Van Beeumen
Dec 10, 1997·Biochimica Et Biophysica Acta·C GerdayG Feller
Dec 31, 1997·Cellular and Molecular Life Sciences : CMLS·G Feller, C Gerday
Oct 7, 1998·Protein Science : a Publication of the Protein Society·J LiangC Woodward
Oct 31, 1998·Protein Engineering·I N Shindyalov, P E Bourne

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Citations

Dec 10, 2002·Proteins·Alessandro PaiardiniStefano Pascarella
Jun 20, 2007·Extremophiles : Life Under Extreme Conditions·Steinar ThorvaldsenNils P Willassen
Jan 20, 2007·Journal of Molecular Modeling·Vojtech SpiwokBlanka Králová
Jun 14, 2005·Biochimica Et Biophysica Acta·Moeava Tehei, Giuseppe Zaccai
May 27, 2003·Bioresource Technology·D R KashyapS K Sharma
Oct 20, 2005·Journal of Bioscience and Bioengineering·Anne HoyouxCharles Gerday
Jun 29, 2011·Biochemistry·Michael HayleyDavid H Heeley
Dec 16, 2011·Journal of Chemical Information and Modeling·Kuei-Jen Lee
Mar 24, 2004·Nature Reviews. Microbiology·Georges Feller, Charles Gerday
Jun 7, 2008·Journal of Biomolecular Structure & Dynamics·C ArcangeliV Rosato
Oct 5, 2012·Protein Engineering, Design & Selection : PEDS·Margherita PezzulloGiuseppe Manco
Nov 17, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tomomi FujiiYasuo Hata
Jan 22, 2008·Applied and Environmental Microbiology·Debora Frigi Rodrigues, James M Tiedje
Jan 30, 2007·Journal of Bacteriology·Anne-Marie W TurnerPamela G Jones
Jun 8, 2006·Annual Review of Biochemistry·Khawar Sohail Siddiqui, Ricardo Cavicchioli
Jul 5, 2006·BMC Bioinformatics·Thorsten Schmidt, Dmitrij Frishman
Feb 24, 2005·Bioscience, Biotechnology, and Biochemistry·Yutaka SuzukiShigenori Kanaya
Jul 19, 2012·PloS One·Mohamad Reza GanjalikhanyTahereh Tohidi Moghadam
Oct 31, 2012·International Journal of Molecular Sciences·Caroline Struvay, Georges Feller
Jul 11, 2013·BioMed Research International·Sebastiana Angelaccio
Jan 10, 2014·Protein Engineering, Design & Selection : PEDS·Virender KumarNalam Madhusudhana Rao
Jul 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeremy L EnglandEugene I Shakhnovich
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Michael J LiszkaDouglas S Clark
Apr 29, 2014·Applied Microbiology and Biotechnology·Pisey M NeangEric Dubreucq
Oct 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Netsanet Gizaw AssefaElin Moe
Jul 5, 2011·Biochimica Et Biophysica Acta·Robert C C MercerDavid H Heeley
Nov 16, 2010·Biophysical Chemistry·Alessandro SiglioccoloStefano Pascarella
Jul 6, 2010·Protein Expression and Purification·Zhisheng LuYu Ding
Nov 10, 2009·Computational Biology and Chemistry·Tienhsiung KuNaiwan Hsiao
Oct 10, 2009·International Journal of Biological Macromolecules·Alessandro SiglioccoloStefano Pascarella
May 12, 2009·International Journal of Biological Macromolecules·Fulvio Baldasseroni, Stefano Pascarella
Nov 7, 2006·Journal of Molecular Graphics & Modelling·Elena PapaleoBjørn O Brandsdal

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