Comparative structure analysis of tyrosine and valine residues in unprocessed silk fibroin (silk I) and in the processed silk fiber (silk II) from Bombyx mori using solid-state (13)C,(15)N, and (2)H NMR

Biochemistry
Tetsuo AsakuraRaghuvansh Kishore

Abstract

The solid-state (13)C CP-MAS NMR spectra of biosynthetically labeled [(13)C(alpha)]Tyr, [(13)C(beta)]Tyr, and [(13)C(alpha)]Val silk fibroin samples of Bombyx mori, in silk I (the solid-state structure before spinning) and silk II (the solid-state structure after spinning) forms, have been examined to gain insight into the conformational preferences of the semicrystalline regions. To establish the relationship between the primary structure of B. mori silk fibroin and the "local" structure, the conformation-dependent (13)C chemical shift contour plots for Tyr C(alpha), Tyr C(beta), and Val C(alpha) carbons were generated from the atomic coordinates of high-resolution crystal structures of 40 proteins and their characteristic (13)C isotropic NMR chemical shifts. From comparison of the observed Tyr C(alpha) and Tyr C(beta) chemical shifts with those predicted by the contour plots, there is strong evidence in favor of an antiparallel beta-sheet structure of the Tyr residues in the silk fibroin fibers. On the other hand, Tyr residues take a random coil conformation in the fibroin film with a silk I form. The Val residues are likely to assume a structure similar to those of Tyr residues in silk fiber and film. Solid-state (2)H NMR me...Continue Reading

References

Jun 1, 1994·Journal of Molecular Evolution·K MitaT C James
Apr 23, 1999·Journal of Biomolecular NMR·M IwadateM P Williamson
May 26, 1999·International Journal of Biological Macromolecules·Y TakahashiK Yuzuriha
May 26, 1999·International Journal of Biological Macromolecules·T AsakuraM P Williamson
Jan 1, 1955·Biochimica Et Biophysica Acta·R E MARSHL PAULING

❮ Previous
Next ❯

Citations

Jun 13, 2013·Acta Biomaterialia·Sangram K SamalEmo Chiellini
Apr 2, 2010·Physical Chemistry Chemical Physics : PCCP·Shailesh NagarkarAshish Lele
Jul 27, 2002·Protein Science : a Publication of the Protein Society·Tetsuo AsakuraYasumoto Nakazawa
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Tetsuo Asakura, Juming Yao
May 5, 2012·Photochemistry and Photobiology·Caroline SolazzoPaul Wyeth
Mar 8, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Tetsuo AsakuraJeffery L Yarger
Jul 20, 2007·Journal of Biomedical Materials Research. Part a·Feng Xin-XingZhang Hua-Peng
Apr 17, 2007·Proteins·Xin ChenFritz Vollrath
Jul 25, 2006·International Journal of Biological Macromolecules·Xin-Xing FengChang-Ian Jia
Feb 14, 2015·International Journal of Biological Macromolecules·Jinfa MingBaoqi Zuo
Nov 26, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Jun-Ting WangQing Shen
Jun 27, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Megan K DeBari, Rosalyn D Abbott
Aug 4, 2018·Macromolecular Rapid Communications·Meghan McGillDavid L Kaplan
Aug 1, 2019·Biomaterials Science·Anastasiia KashirinaJinsong Leng
Apr 12, 2014·Biopolymers·Guoqing LiuXinwei Wang
Oct 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Danielle L HeichelKelly A Burke
Feb 18, 2021·The Journal of Physical Chemistry. B·Romeo C A DubiniPetra Rovó
Jul 11, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Rajkamal BaluNamita Roy Choudhury
May 14, 2003·Biomacromolecules·Tetsuo AsakuraDavid L Kaplan
Dec 1, 2005·Analytical Chemistry·Bing-Wen HuGuang-Zhi Zhao
Dec 9, 2021·Archives of Insect Biochemistry and Physiology·Shanshan TaoChongjun Ye

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.