Comparative studies of mesenchymal stem cells derived from different cord tissue compartments - The influence of cryopreservation and growth media

Placenta
Magda DulugiacOtilia Zarnescu

Abstract

We have identified some critical aspects concerning umbilical cord tissue mesenchymal stem cells: the lack of standards for cell isolation, expansion and cryopreservation, the lack of unanimous opinions upon their multilineage differentiation potential and the existence of very few results related to the functional characterization of the cells isolated from cryopreserved umbilical cord tissue. Umbilical cord tissue cryopreservation appears to be the optimal solution for umbilical cord tissue mesenchymal stem cells storage for future clinical use. Umbilical cord tissue cryopreservation allows mesenchymal stem cells isolation before expected use, according with the specific clinical applications, by different customized isolation and expansion protocols agreed by cell therapy institutions. Using an optimized protocol for umbilical cord tissue cryopreservation in autologous cord blood plasma, isolation explant method and growth media supplemented with FBS or human serum, we performed comparative studies with respect to the characteristics of mesenchymal stem cells (MSC) isolated from different compartments of the same umbilical cord tissue such as Wharton's jelly, vein, arteries, before cryopreservation (pre freeze) and after cry...Continue Reading

References

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Citations

Sep 17, 2018·Stem Cell Research & Therapy·Irina ArutyunyanGennady Sukhikh
Nov 14, 2018·Frontiers in Pharmacology·Gina D KusumaRebecca Lim
Jun 27, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Panagiotis MallisEfstathios Michalopoulos

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