Comparative studies of Toxoplasma gondii transcriptomes: insights into stage conversion based on gene expression profiling and alternative splicing

Parasites & Vectors
Longfei ChenXiao-Guang Chen

Abstract

Toxoplasma gondii is one of the most important apicomplexan parasites and infects one-third of the human population worldwide. Transformation between the tachyzoite and bradyzoite stages in the intermediate host is central to chronic infection and life-long risk. There have been some transcriptome studies on T. gondii; however, we are still early in our understanding of the kinds and levels of gene expression that occur during the conversion between stages. We used high-throughput RNA-sequencing data to assemble transcripts using genome-based and de novo strategies. The expression-level analysis of 6996 T. gondii genes showed that over half (3986) were significantly differentially expressed during stage conversion, whereas 2205 genes were upregulated, and 1778 genes were downregulated in tachyzoites compared with bradyzoites. Several important gene families were expressed at relatively high levels. Comprehensive functional annotation and gene ontology analysis revealed that stress response-related genes are important for survival of bradyzoites in immune-competent hosts. We compared Trinity-based de novo and genome-based strategies, and found that the de novo assembly strategy compensated for the defects of the genome-based str...Continue Reading

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Citations

Nov 24, 2018·F1000Research·Martin Blume, Frank Seeber
Jul 7, 2020·Frontiers in Cellular and Infection Microbiology·Martin J McPhillieRima McLeod
Aug 22, 2020·Emerging Microbes & Infections·Hyang-Hee SeoJung-Hyun Kim
Feb 21, 2019·MBio·Lee M YeohStuart A Ralph
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Sarah L Sokol-BorrelliJon P Boyle

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Methods Mentioned

BETA
RNA-Seq
density gradient centrifugation
glycosylation

Software Mentioned

Blastx
PASA SPLICE
Tophat
Blast2GO
Chrysalis
TopHat2
ToxoDB
Trinity assembly suite
MEME
Integrative Genomics Viewer ( IGV )

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