Comparative studies on bull and stallion seminal DNase activity and interaction with semen extender and spermatozoa

Animal Reproduction Science
Abdorrahman S AlghamdiDouglas N Foster

Abstract

We performed a series of comparative studies of bull and stallion seminal plasma (SP) and its role on sperm-neutrophil binding as well as the interaction between semen extender and seminal DNase. Because of contrasting roles of SP on sperm-neutrophil binding between horses and cattle, it was suspected there were some species-specific differences on sperm interaction with SP proteins due to the variations in the natural location of semen deposition (uterus compared to vagina). Bull frozen-thawed sperm removed from egg yolk extender showed similar results to fresh sperm, but this also caused extensive sperm agglutination unless SP or egg yolk was included. If similar agglutination occurs after AI with frozen bull semen, it could interfere with sperm transport or sperm functions. Commonly used bull semen extenders were poor media for seminal DNase activity on plasmid DNA degradation, raising the prospect that the same may be true with other SP factors important to fertility. DNase activity per mg SP protein of bulls was less than that of horses (P<0.05), but DNase activity associated with bull sperm was greater (P<0.05) indicating a different affinity of DNase to spermatozoa. This could be related to the fact that bull sperm natur...Continue Reading

References

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Dec 17, 2008·Animal Reproduction Science·Abdorrahman S AlghamdiDouglas N Foster

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Citations

Sep 16, 2014·Reproduction in Domestic Animals = Zuchthygiene·J E Schjenken, S A Robertson
Sep 16, 2016·Journal of Assisted Reproduction and Genetics·Larissa Berloffa BelardinAgnaldo Pereira Cedenho
Jan 18, 2018·Molecular Reproduction and Development·Ahmed E ElwezaAkio Miyamoto
Jan 31, 2020·Physiological Reviews·John E Schjenken, Sarah A Robertson
Apr 29, 2021·Equine Veterinary Journal·Yentel Mateo-OteroBeatriz Fernandez-Fuertes

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