PMID: 44526Nov 1, 1979

Comparative studies on dihydrofolate reductases from Plasmodium falciparum and Aotus trivirgatus

The Journal of protozoology
S C Kan, W A Siddiqui

Abstract

Dihydrofolate reductase (E.C. 1.5.1.3) from Plasmodium falciparum and from its host, the owl monkey (Aotus trivirgatus), were partially purified and characterized. The molecular weight of the parasite enzyme was estimated to be over 10 times as high as that of the host enzyme. The host enzyme had 2 pH optima whereas the parasite enzyme only one. The activity of the host enzyme was greatly stimulated by KCl and urea, while that of the parasite enzyme was inhibited at high concentrations of such chaotropic agents. Km of the parasite enzyme was significantly higher than that of the host enzyme. The parasite enzyme had much lower Ki for pyrimethamine than the host enzyme. Dihydrofolate reductases isolated from pyrimethamine-resistant and pyrimethamine sensitive strains of P. falciparum were found to be similar.

References

Jun 1, 1973·Annals of Tropical Medicine and Parasitology·W H Richards, S G Williams
May 1, 1974·The Journal of protozoology·E G Platzer
Jun 1, 1971·Parasitology·W E Gutteridge, P I Trigg
Aug 1, 1953·The Biochemical Journal·M DIXON

Citations

Oct 1, 1984·International Journal for Parasitology·S N Pattanakitsakul, P Ruenwongsa
Oct 1, 1986·International Journal for Parasitology·A Ratanaphan, P Ruenwongsa
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·W M WatkinsR E Howells
Jan 1, 1990·Pharmacology & Therapeutics·W Peters
Apr 1, 1986·Molecular and Biochemical Parasitology·R D Walter
Jan 1, 1989·Molecular and Biochemical Parasitology·J KrungkraiY Yuthavong
Aug 1, 1991·Molecular and Biochemical Parasitology·T FandeurO Mercereau-Puijalon
Oct 1, 1986·Parasitology·A Dieckmann, A Jung
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A F CowmanS J Foote
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D S PetersonT E Wellems
Nov 1, 1984·Antimicrobial Agents and Chemotherapy·T F McCutchanC J Allegra
Apr 1, 1985·Antimicrobial Agents and Chemotherapy·W K MilhousR E Desjardins
Dec 21, 2010·Trends in Parasitology·Xavier C DingGiovanna Raso
Dec 1, 1981·Experimental Parasitology·J GolenserY Pollack
Nov 10, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·W Sirawaraporn
Jan 1, 1981·Medicinal Research Reviews·J J McCormack
Mar 1, 2005·Pharmacological Reviews·Aric Gregson, Christopher V Plowe
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A P RobertsonR J Martin

Related Concepts

Anthropoidea
Night Monkey, Northern
Erythrocytes
Hydrogen-Ion Concentration
Malaria
Plasmodium falciparum
Slow-K
Malocide
Tetrahydrofolate Dehydrogenase
Carmol

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