Comparative studies on interactions of bovine serum albumin with cationic gemini and single-chain surfactants

The Journal of Physical Chemistry. B
Yajuan LiYilin Wang

Abstract

The interactions of bovine serum albumin (BSA) with cationic gemini surfactants alkanediyl-alpha,omega-bis(dodecyldimethylammonium bromide) [C12H25(CH3)2N(CH2)(S)N(CH3)2C12H25]Br2 (designated as C12C(S)C12Br2, S = 3, 6, and 12) and single-chain surfactant dodecyltrimethylammonium bromide (DTAB) have been studied with isothermal titration microcalorimetry, turbidity, fluorescence spectroscopy, and circular dichroism at pH 7.0. Comparing with DTAB, C12C(S)C12Br2 have much stronger binding ability with BSA to induce the denaturation of BSA at very low molar ratio of C12C(S)C12Br2/BSA, and C12C(S)C12Br2 have a much stronger tendency to form insoluble complexes with BSA. The binding of C12C(S)C12Br2 to BSA generates larger endothermic peaks. The first endothermic peak is much stronger than that of the second endothermic peak. The double charges and strong hydrophobicity of the gemini surfactants are the main reasons for these observations. In addition, the spectra results show that the binding of DTAB to BSA only promotes BSA unfolding and aggregation, whereas the secondary structure of BSA is possibly stabilized by a small amount of C12C(S)C12Br2 , even if the small amount of binding C12C(S)C12Br2 could induce the loss of the terti...Continue Reading

References

Dec 15, 1994·European Journal of Biochemistry·D C CarterZ Krishnasami
Jun 1, 1997·International Journal of Biological Macromolecules·C GiancolaG Barone
Nov 1, 2000·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·E L Gelamo, M Tabak
Jan 1, 1955·Biochimica Et Biophysica Acta·A V FEW
Jun 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Apiradee WangsakanDavid J McClements

❮ Previous
Next ❯

Citations

May 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Soumyananda ChakrabortyPinak Chakrabarti
Sep 11, 2007·Toxicon : Official Journal of the International Society on Toxinology·Shu-Huei TsaiInn-Ho Tsai
Jul 15, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Mengyao HuGongwu Song
Sep 29, 2015·Colloid and Polymer Science·W Gospodarczyk, M Kozak
Jul 15, 2015·Applied Biochemistry and Biotechnology·Nisar Ahmad Malik
Nov 1, 2016·ACS Applied Materials & Interfaces·Chengcheng ZhouYilin Wang
Apr 1, 2017·International Journal of Biological Macromolecules·Nuzhat GullRizwan Hassan Khan
Apr 8, 2015·Chemical Communications : Chem Comm·Lu XuJingcheng Hao
Aug 8, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·G VigneshK Premkumar
Jan 13, 2011·Physical Chemistry Chemical Physics : PCCP·Yuchun Han, Yilin Wang
Jul 23, 2011·Biopolymers·Hiroaki KitagishiKoji Kano
Dec 25, 2019·International Journal of Biological Macromolecules·Rachana Srivastava, Md Sayem Alam
Feb 13, 2010·The Journal of Physical Chemistry. B·Mohammad Amin MirGhulam Mohammad Rather
Jul 30, 2009·Biomacromolecules·Célia M C FaustinoLuís Garcia-Rio
Apr 8, 2015·International Journal of Biological Macromolecules·Mohd Akram Kabir-ud-Din
Apr 10, 2016·International Journal of Biological Macromolecules·Mohd Akram Kabir-Ud-Din
Oct 26, 2018·International Journal of Biological Macromolecules·Rachana Srivastava, Md Sayem Alam
Apr 26, 2012·The Journal of Physical Chemistry. B·Mohammad Amin MirGhulam Mohammad Rather
May 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Victoria Isabel MartínMaría Luisa Moyá
Dec 25, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Mónica Ospinal-Jiménez, Danilo C Pozzo
Dec 21, 2013·The Journal of Physical Chemistry. B·Pankaj BharmoriaArvind Kumar
Feb 13, 2007·Biomacromolecules·Dan WuYujun Feng
Sep 6, 2011·Journal of Colloid and Interface Science·Nuzhat GullAijaz Ahmad Dar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.