Comparative studies on osteogenic potential of micro- and nanofibre scaffolds prepared by electrospinning of poly(ε-caprolactone)

Progress in Biomaterials
Ting-Ting LiThomas Groth

Abstract

The biocompatibility and osteogenic potential of four fibrous scaffolds prepared by electrospinning of poly(ε-caprolactone) (PCL) was studied with MG-63 osteoblast cells. Two different kinds of scaffolds were obtained by adjustment of spinning conditions, which were characterized as nano- or microfibrous. In addition of one nanofibrous, scaffold was made more hydrophilic by blending PCL with Pluronics F 68. Scaffolds were characterized by scanning electron microscopy and water contact angle measurements. Morphology and growth of MG63 cells seeded on the different scaffolds were investigated by confocal laser scanning microscopy after vital staining with fluorescein diacetate and by colorimetric assays. It was found that scaffolds composed of microfibres stipulated better growth conditions for osteoblasts probably by providing a real three-dimensional culture substratum, while nanofibre scaffolds restricted cell growth predominantly to surface regions. Osteogenic activity of cells was determined by alkaline phosphatase (ALP) and o-cresolphthalein complexone assay. It was observed that osteogenic activity of cells cultured in microfibre scaffolds was significantly higher than in nanofibre scaffolds regarding ALP activity. Overall...Continue Reading

References

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Citations

Oct 1, 2016·Journal of Tissue Engineering and Regenerative Medicine·Sharanya SankarSeeram Ramakrishna
Apr 17, 2020·Journal of Applied Biomaterials & Functional Materials·Supaporn SangkertJirut Meesane
Dec 1, 2014·Progress in Biomaterials·Robin AugustineSabu Thomas
May 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Biranche TandonSarah H Cartmell

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Methods Mentioned

BETA
Assay
confocal microscopy

Software Mentioned

Leica Confocal
Image J

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