Comparative studies on prostanoid receptors in human non-pigmented ciliary epithelial and mouse fibroblast cell lines

Prostaglandins, Leukotrienes, and Essential Fatty Acids
L LiuC A Paterson

Abstract

To examine the expression of functional prostanoid receptors in the human non-pigmented ciliary epithelial (ODMC1-2) and mouse fibroblast cell lines (NIH 3T3) we have measured the generation of the second messengers, cyclic AMP, inositol phosphates and the mobilization of intracellular calcium ([Ca2+]i) following stimulation by prostaglandin receptor agonists. The amount of cyclic AMP generated was measured by a protein binding method. Radiolabeled inositol phosphates were separated using ion exchange columns and quantified by counting the radioactivity. For intracellular calcium measurements, Fura 2-AM loaded cells were stimulated by PG receptor agonists and the calcium activated fluorescence was measured in a spectrofluorometer. In the ODMC1-2 cell line, the formation of cyclic AMP was stimulated by prostaglandin E2, butaprost and 11-deoxy-prostaglandin E1. The stimulation of cyclic AMP production by prostaglandin E2 was partially inhibited by the EP4 receptor antagonist AH23848. Prostaglandin E2 and 11-deoxy-prostaglandin E1 stimulated the formation of cyclic AMP in NIH 3T3 cells. In ODMC1-2 cells, total inositol phosphate turnover was not increased by 17-phenyl-trinor-prostaglandin F2 alpha, 17-phenyl-trinor-prostaglandin E...Continue Reading

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Citations

Apr 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J Y Crider, N A Sharif
Apr 30, 2003·Expert Opinion on Biological Therapy·Surajit DeyAshim K Mitra
Oct 21, 2006·Experimental Eye Research·S BiswasS Narumiya
Jun 13, 2006·Ocular Immunology and Inflammation·S BiswasB H Koller
Jul 20, 1999·Ocular Immunology and Inflammation·P BhattacherjeeC A Paterson
May 9, 1997·Biochemical Pharmacology·P MukhopadhyayC A Paterson
Jun 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P BhattacherjeeC A Paterson

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