Comparative studies on the interaction of proteins with a polydimethylsiloxane elastomer. I. Monolayer protein capture capacity (PCC) as a function of protein pl, buffer pH and buffer ionic strength

Journal of Molecular Recognition : JMR
J E ButlerB Christiansen

Abstract

Polydimethylsiloxane (PEP) is widely used in medical prostheses and therefore is in contact with plasma and secretory proteins. Two pair of globular proteins, lactoferrin (Lf) and transferrin (Trf), and bovine IgG1 and IgG2a, which differ substantially between pair members in their pl, were used to study the interaction of a PEP widely used in breast implants and soluble protein. Studies were done using iodinated proteins over a concentration range that resulted in an apparent protein monolayer. Secondary incubations with dilute protein solutions were needed to form the monolayer on PEP, possibly as a consequence of micro air bubbles trapped on its highly textured surface as shown by atomic force microscopy. Immunoassay quality polystyrene microtiter wells were used as controls. Adsorption studies were routinely performed at pH 4, 7 and 10 and at ionic strengths corresponding to 0.95, 9.5 and 90.0 mS. The protein capture capacity (PCC) of PEP for Lf and Trf was optimal at physiological pH and ionic strength and comparable under these conditions to that of Immulon 2 (Imm 2) microtiter wells. While increasing the ionic strength and pH further increases the PCC of Imm 2 for Lf and Trf, this markedly lowered the PCC of PEP for thes...Continue Reading

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Citations

Mar 29, 2003·Journal of Biomaterials Science. Polymer Edition·Magdalena ProkopowiczAndrzej Przyjazny
Jan 1, 2012·Biomicrofluidics·S Forster, S L McArthur
Jan 1, 2009·Biomicrofluidics·Walter SchrottDalimil Snita
Jul 22, 2004·Analytical Biochemistry·Lasse VälimaaTimo Lövgren
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sutapa BaruaSamir Mitragotri
Sep 30, 1999·Environmental Health Perspectives·V M ShaheenW H Reeves
Jan 5, 2007·Lab on a Chip·Michael W Toepke, David J Beebe
Dec 7, 2007·Electrophoresis·Youyuan PengMyriam Taverna
Nov 13, 2008·Analytical and Bioanalytical Chemistry·Aristidis E NiotisSotirios E Kakabakos
Oct 6, 2000·Methods : a Companion to Methods in Enzymology·J E Butler

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