Comparative studies on vascular endothelium in vitro. 2. Hypoxia: its influences on endothelial cell proliferation and expression of cell adhesion molecules

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
C L KleinC J Kirkpatrick

Abstract

Recent studies have presented evidence that the processes of hypoxaemia and reperfusion are involved in several pathogenetic mechanisms of atherosclerotic lesions. The ability of hypoxaemia to activate circulating white blood cells (WBCs) and enhance WBC-endothelial cell (EC) interactions is suspected to be a major factor in deleterious processes in the blood vessel wall. Various groups have suggested that cell adhesion molecules (CAMs), such as ICAM-1, VCAM-1 and E-selectin and their leukocyte ligands are involved in intercellular activities of the relevant cell types. We studied the effects of different oxygen tensions, simulating normoxic conditions, hypoxia and hyperoxia in vitro with the help of an umbilical vein EC model in order to determine the effects of oxygenation on CAM presentation on vascular ECs with and without further cytokine and endotoxin (lipopolysaccharides; LPS) stimulation. Semiquantitative analysis of ICAM-1, E-selectin and VCAM-1 was performed using cell enzyme immunoassay techniques. The presentation of ICAM-1, E-selectin and VCAM-1 remained on the whole unaffected by both hypoxia and hyperoxic conditioning after both 7 and 24 h. Stimulation of ICAM-1 by cytokines and LPS was only marginally influenced...Continue Reading

Citations

Sep 30, 1996·Annals of the New York Academy of Sciences·V F BrocheE Prieto
Jul 11, 2007·Der Urologe. Ausg. A·H SperlingH Rübben
Aug 15, 2013·Psychotherapy and Psychosomatics·Andreas BaranyiHans-Bernd Rothenhäusler
Jun 23, 2011·Therapeutic Advances in Cardiovascular Disease·David J PerkowskiJ A St Cyr
Mar 26, 1998·Journal of Cardiovascular Pharmacology·T ArnouldJ Remacle
Feb 24, 2000·The Journal of Pathology·H A LehrC J Kirkpatrick

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