PMID: 2488893Sep 1, 1989Paper

Comparative study of androgen binding in rat submandibular gland and prostate

The Bulletin of the Kanagawa Dental College : BKDC
R Yorichika

Abstract

A comparative study of androgen binding in the cytosol and nuclear extract of the rat submandibular gland (SMG) and ventral prostate (VP) was investigated by using methyltrienolone (R1881), testosterone (T) and dihydrotestosterone (DHT) as the labeled ligands. The 3H-labeled steroid was incubated with the cytosol or nuclear KCl extract in a Tris-HCl buffer (pH 7.4) at 4 degrees C for 20 h. A sephacryl S-200 column chromatography showed a single binding peak in the nuclei and two critical peaks on the binding in the cytosol of SMG. The eluation patterns of SMG were similar to those of the nuclei and cytosol from rat VP. The binding proteins were recovered predominantly in the cytosol as 150-200 kDa mol wt and in the nuclei as 14-20 Kda mol wt. The cytosol and nuclei of rat SMG and VP have a common androgen binding subunit of the molecular weight of 14-20 kDa. R1881 bound to cytosolic protein with a higher affinity than DHT in SMG and VP. The amount of the R1881 specific binding in the cytosol of male SMG was similar to that of VP, and the DHT specific binding in the male SMG cytosol was about 1/3 of the binding in the cytosol of VP. R1881, DHT and T suppressed the 3H-R1881 binding completely in the cytosol of rat VP. Whereas, in...Continue Reading

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