Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus

Journal of Bioscience and Bioengineering
Honghai HuNorio Sugiura

Abstract

The medicinal mushroom Inonotus obliquus is a traditional and widely used multi-functional fungus. Hot water (50 degrees C, 70 degrees C, and 80 degrees C) and ethanol crude extracts of I. obliquus were investigated for their antioxidant activity with superoxide dismutase (SOD) and (1,1-diphenyl-2-picryhydrazyl) (DPPH) radical-scavenging activity assays. We also investigated the antiproliferative effects and ability of the extracts to induce apoptosis in human colon cancer DLD-1 cells. Among the four extracts, the ethanol extract (EE) exhibited the strongest SOD-like activity and antiproliferative effect on DLD-1 cells, and exposure to the EE resulted in the induction of apoptosis, whereas no apoptosis was observed in DLD-1 cells exposed to the hot water extracts (HWEs). HWE at 70 degrees C (HWE70) exhibited the strongest DPPH radical-scavenging activity (EC50, 126 microg/ml), whereas the EE showed the weakest activity (EC50, 224 microg/ml). The different biological activities among the four extracts may be attributed to differences in their chemical composition, partially supported by polysaccharide, protein and phenolic content, and the 1H-NMR spectra.

References

Feb 1, 1991·Journal of Ethnopharmacology·M Saar
Dec 1, 1990·Biochemical Society Transactions·K F Gey
Aug 7, 1997·The New England Journal of Medicine·M N DiazJ F Keaney
Sep 19, 1998·Free Radical Biology & Medicine·G L Squadrito, W A Pryor
Jan 19, 2000·Clinical Biochemistry·J M MatésI Núñez de Castro
Nov 19, 2002·Applied Microbiology and Biotechnology·S P Wasser
Dec 14, 2004·Journal of Ethnopharmacology·Yong CuiKyoung-Chan Park
Dec 1, 1986·Planta medica·K KahlosR Hiltunen
Aug 2, 2007·Chemical & Pharmaceutical Bulletin·Yuki NakajimaTetsuya Konishi

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Citations

Sep 4, 2012·International Journal of Molecular Sciences·Marcelo Gonzaga de Freitas AraújoTais Maria Bauab
May 15, 2012·3 Biotech·Seema Patel, Arun Goyal
Jan 13, 2015·Journal of Ethnopharmacology·Jasmina GlamočlijaLeo J L D van Griensven
Aug 20, 2014·Journal of Medicinal Food·Yvonne C O'CallaghanThomas J Smyth
Jan 15, 2015·International Journal of Biological Macromolecules·Qingjie WangJinyou Duan
Jul 1, 2014·Journal of Asian Natural Products Research·He-Ping ChenJi-Kai Liu
Jun 28, 2017·International Journal of Molecular Sciences·Ying NieFeijun Luo
Oct 11, 2019·Journal of Food Biochemistry·Ebru DeveciMehmet Öztürk
May 18, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Fu-Qiang SongGe Song
Aug 21, 2019·International Journal of Molecular Sciences·Adriana FontesAnabela Marisa Azul
Jul 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiao-Jiao ZhangHua-Bin Li
Aug 27, 2015·World Journal of Gastroenterology : WJG·Yu-Hua LiQi-Bing Mei
Jan 1, 2011·Experimental and Therapeutic Medicine·DI GuanYing Zhang
Jul 2, 2021·Journal of Traditional and Complementary Medicine·Konrad A SzychowskiJan Gmiński

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