Comparative study of biochemical methods for the identification of Dichelobacter nodosus

Acta Veterinaria Hungarica
Ruth JiménezS Vadillo

Abstract

The aim of this study was to compare four identification procedures to detect Dichelobacter nodosus and develop a rapid, simple and effective method to identify D. nodosus strains isolated from cases of ovine footrot. The four methods used were: (a) the classic guidelines set down by Holdeman et al. (1977) and Summanen et al. (1993) which are based on gas liquid chromatography (GLC) and different biochemical tests, this method was considered as landmark; (b) Baron and Citron's flowchart for the rapid identification of Gram-negative rod-shaped anaerobes (1997); (c) the API rapid 32 A system (bio Mérieux), and (d) Mast ID Anaerobe ID Ring (MID8) (Mast Diagnostics). None of the four methods used allowed us to correctly identify the D. nodosus strains (neither the strains isolated from cases of ovine footrot nor those originating from type collection). Because of the difficulties encountered in obtaining a correct identification of D. nodosus, we propose a simple, rapid and effective way to achieve this task. Our flowchart will provide the means to identify this microorganism in any laboratory of general microbiology without having to use any specialised equipment.

References

Sep 1, 1991·Journal of Clinical Microbiology·S PirizS Vadillo
Mar 1, 1985·Antimicrobial Agents and Chemotherapy·V L SutterJ Bulkacz
Jun 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Summanen
May 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Summanen
Oct 6, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E J Baron, D M Citron
Jun 14, 2001·Acta Veterinaria Hungarica·S PírizS Vadillo
Apr 2, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·R JiménezS Vadillo

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