PMID: 6967559Jan 1, 1980Paper

Comparative study of biological activities and mitogenic effect of extracts from Haemophilus influenzae type a

Medical Microbiology and Immunology
D RaichvargJ E Alouf

Abstract

Two preparations designated as PWE and NCE were obtained from H, influenzae type a by treatment with either phenol water or hypertonic NaCl citrate respectively. The protein content of NCE was three fold higher than for PWE. Electroimmunodiffusion against H. influenzae antiserum disclosed an immunological relationship between NCE and PWE. The toxicity for mice was lower for both antigenic extracts (LD50 PWE: 47 +/- 7 mg/kg; LD50 NCE: 943 +/- 5 mg/kg) than for E. coli LPS. Limulus assay showed that both H. influenzae extracts had a weaker potency (100 to 1000 times less) than E. coli LPS. Sanarelli and Shwartzman reactions failed for PWE and NCE. The blast transformation of DBA/2 and nude mouse spleen cells were strikingly different for the three preparations: PWE, NCE, and E. coli LPS. Neither extract from H. influenzae cells showed the biological properties of a classical endotoxin. We suggest that they are endotoxin-like substances.

References

Nov 1, 1978·Die Naturwissenschaften·O LüderitzJ Weckesser
Jan 1, 1976·Annual Review of Medicine·R J Elin, S M Wolff
Aug 1, 1976·Japanese Journal of Microbiology·M Kanamori
Jan 1, 1974·Methods in Enzymology·D M Livingston
Jul 1, 1973·The Journal of Infectious Diseases·M RaynaudA Petitprez
Oct 1, 1968·Canadian Journal of Biochemistry·G A AdamsM Yaguchi
Jan 1, 1969·Antonie van Leeuwenhoek·H O Eggins, K A Malik
Dec 10, 1970·The New England Journal of Medicine·J LevinR S Oser

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