Comparative study of carbohydrate portion of gastrointestinal mucins using enzyme-linked lectin-binding assay (ELLA)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
S OharaK Hotta

Abstract

We improved the enzyme-linked lectin-binding assay (ELLA) to determine the differences in the carbohydrate chains of corpus, antral, duodenal ant colonic rat mucins. First we have improved the optimal conditions of this assay for mucins; ELLA makes possible the detection of 1.5 ng of hexose in rat gastrointestinal mucins (5-7 ng of mucins). Salt concentrations of several dozens mM are required for mucin coating on the plate. Non-ionic detergents diminish the adsorption of mucins onto the plate. Secondly we tested a set of 8 lectins to compare their binding to the gastrointestinal mucin samples. It is possible to detect crude mucins as well as purified mucins using ELLA. Gastric mucins have less Tn-antigen than duodenal and colonic mucins. Corpus and duodenal mucins have more of the H-type 2 chain than antral and colonic mucins.

References

Jun 1, 1976·The American Journal of Anatomy·D G Sheahan, H R Jervis
Dec 11, 1992·American Journal of Respiratory Cell and Molecular Biology·J R Gum
Jun 1, 1988·Journal of Biochemistry·S OharaK Hotta
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S OharaK Hotta
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S OharaK Hotta
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S OharaK Hotta

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Citations

Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T FujitaK Hotta
Oct 2, 2012·Biochemical Pharmacology·Shintaro SuemasuTohru Mizushima
Oct 5, 2014·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Flavia LiottaLudovico Pontoni

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