PMID: 2118899Jul 1, 1990Paper

Comparative study of cell-immunoenzymatic methods for the estimation of IgG and IgM anti-Brucella antibodies in the diagnosis of human brucellosis

The Journal of Applied Bacteriology
C MonginiS E Hajos

Abstract

Two methods which employ whole cells are described and compared for the detection of human IgG and IgM anti-brucella antibodies. Dot ELISA and ELISA were shown to be suitable for a screening diagnosis of human brucellosis. Titres of antibodies obtained by dot ELISA showed 100% coincidence for IgG and 97% for IgM, compared with agglutination and complement fixation tests; when ELISA was used 11% positive sera were not detected. The comparison of these two methods with the conventional serological test kit indicated that both dot ELISA and ELISA were sensitive, reproducible and specific for the quantification of IgG and IgM antibrucella antibodies.

References

May 21, 1977·Lancet·D ParrattR G White
Feb 11, 1987·Journal of Immunological Methods·H BaumgartenO Götze
May 1, 1986·Journal of Immunological Methods·M Lefroit-Joliy, T Neveu
Jan 1, 1986·The Journal of Infectious Diseases·E GotuzzoL Llosa
Dec 1, 1985·The Journal of Hygiene·W HeizmannK Fleischmann
Mar 1, 1968·Public Health·W J Patterson
Sep 1, 1983·Reviews of Infectious Diseases·E J Young
Feb 1, 1980·Journal of Medical Microbiology·J T Magee
Jun 1, 1980·Journal of Clinical Microbiology·T M Buchanan, L C Faber
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Jun 17, 1965·The New England Journal of Medicine·J L REDDINW W SPINK

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Citations

Jan 1, 1996·Parasitology·D P ThompsonT G Geary
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·W E Hill

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