PMID: 8938951Jan 1, 1996Paper

Comparative study of classical, colorimetric and immunologic staining methods for the assessment of tumor cell viability

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
G BashaF Penninckx

Abstract

The trypan blue exclusion test, the MTT test and an immunostaining test for apoptosis were performed before and after incubation of SW620 human colonic carcinoma cells with different cytotoxic agents (CTAs) in order to assess tumor cell viability and CTA efficacy in vitro. A modified MTT test using light microscopy was also performed. A good correlation was found between the trypan blue assay and the MTT test, as determined by spectrophotometry. There was no 'overestimation' of cell viability as measured by the trypan blue test. The monitoring of formazan formation by light microscopy was feasible, but not very reliable since it did not show a good correlation with findings determined by spectrophotometry. The apoptosis test failed to show good correlation with other tests. Distilled water had no relevant cytotoxic effect, while chlorhexidine cetrimide (HAC 3.5%), chloramine 0.5% and polyvinylpyrrolidone iodine (PVP-I) > or = 0.05% damaged a large majority of cells. PVP-I at a concentration of > or = 5% was found to be the most effective CTA.

Citations

Apr 21, 2010·The Journal of Physiology·José Linares-PalominoManuel Galiñanes
Feb 15, 2011·The Journal of Surgical Research·Mohammad Ali HusainyManuel Galiñanes
Nov 10, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·François DepaultNicole Lemieux
Mar 14, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P WeybrightS Singer
Sep 28, 1998·The Annals of Otology, Rhinology, and Laryngology·P Cayé-Thomasen, M Tos
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y HoshinoT Izumi

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