Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations.

Genetics Research International
Masahiko ImashimizuNobuo Shimamoto

Abstract

If Mg(2+) ion is replaced by Mn(2+) ion, RNA polymerase tends to misincorporate noncognate nucleotide, which is thought to be one of the reasons for the toxicity of Mn(2+) ion. Therefore, most cells have Mn(2+) ion at low intracellular concentrations, but cyanobacteria need the ion at a millimolar concentration to maintain photosynthetic machinery. To analyse the mechanism for resistance against the abundant Mn(2+) ion, we compared the properties of cyanobacterial and E. coli RNA polymerases. The cyanobacterial enzyme showed a lower level of abortive transcription and less misincorporation than the E. coli enzyme. Moreover, the cyanobacterial enzyme showed a slower rate of the whole elongation by an order of magnitude, paused more frequently, and cleaved its transcript faster in the absence of NTPs. In conclusion, cyanobacterial RNA polymerase maintains the fidelity of transcription against Mn(2+) ion by deliberate incorporation of a nucleotide at the cost of the elongation rate. The cyanobacterial and the E. coli enzymes showed different sensitivities to Mg(2+) ion, and the physiological role of the difference is also discussed.

References

Oct 1, 1990·Genes & Development·J P Jacques, M M Susskind
Jul 1, 1989·Journal of Bacteriology·M R Schaefer, S S Golden
Sep 1, 1988·Journal of Bacteriology·G J Schneider, R Hasekorn
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G HindS Izawa
Aug 7, 1972·Biochemical and Biophysical Research Communications·J HurwitzS Wickner
Jan 1, 1981·Toxicology·S K NiyogiD J Hoffman
Jun 5, 1983·Journal of Molecular Biology·J J Dunn, F W Studier
Jun 11, 1981·Nucleic Acids Research·S K Niyogi, R P Feldman
Mar 1, 1996·Journal of Molecular Biology·T Kubori, N Shimamoto
Oct 1, 1989·Physical Review A: General Physics·A BlumenJ Klafter
Feb 7, 2001·The Journal of Biological Chemistry·R SenN Shimamoto
May 31, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·R SenN Shimamoto
Jan 1, 1987·Plant Physiology·S BelkinL Packer
May 3, 2003·The EMBO Journal·Vasily SosunovAlex Goldfarb
Feb 1, 2005·Cell·Gil Bar-NahumEvgeny Nudler
Nov 16, 2005·Molecular Cell·Marni RaffaelleAseem Z Ansari
Mar 24, 2006·Molecular Microbiology·Motoki SusaNobuo Shimamoto
Oct 21, 2006·Molecular Cell·Richard T PomerantzWilliam T McAllister
Oct 21, 2006·Molecular Cell·Ekaterina KashkinaDmitry Temiakov
Mar 22, 2007·PLoS Biology·Michael J DalyJames K Fredrickson
Sep 4, 2007·Journal of Bacteriology·Ekaterina StepanovaSergei Borukhov
May 15, 2009·The Journal of Biological Chemistry·Celine WalmacqMikhail Kashlev
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi ItoHideo Iwasaki

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Citations

Aug 13, 2015·Biotechnology and Bioengineering·Cheryl M ImmethunTae Seok Moon
Apr 22, 2015·Nucleic Acids Research·Bin ZhuCharles C Richardson
Sep 18, 2012·Biochimica Et Biophysica Acta·Vladimir Svetlov, Evgeny Nudler
Jan 29, 2014·Journal of Biological Engineering·Daniel CamsundPeter Lindblad
Oct 18, 2014·Frontiers in Bioengineering and Biotechnology·Daniel Camsund, Peter Lindblad
Aug 9, 2013·Nucleic Acids Research·Masahiko ImashimizuMikhail Kashlev
Dec 22, 2012·The Journal of Biological Chemistry·Bin ZhuCharles C Richardson
Mar 7, 2019·Biochemical Society Transactions·Amber Riaz-Bradley

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Methods Mentioned

BETA
PCR
Hi-trap
cleavage assay
Assay
chemical
on in vitro

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