Comparative study of cytochrome P-450 in liver microsomes. A form of monkey cytochrome P-450, P-450-MK1, immunochemically cross-reactive with antibodies to rat P-450-male

Biochemical Pharmacology
H OhiT Kamataki

Abstract

Cytochrome P-450, designated as P-450-MK1, which is cross-reactive with antibodies to rat P-450-male, was purified to an electrophoretical homogeneity from liver microsomes of the untreated male crab-eating monkey. The molecular weight of P-450-MK1 was estimated to be 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The oxidized form of P-450-MK1 showed a peak at 418 nm, indicating that this cytochrome is in a low spin state. The carbon monoxide-bound reduced form showed a peak at 451 nm. The first 22 amino acid residues of the NH2-terminal sequence of P-450-MK1 was fairly homologous to those of P-450-male (75% identity, not including unidentified amino acid residues). Unlike the P-450-male, P-450-MK1 did not exhibit catalytic activities for testosterone 2 alpha- and 16 alpha-hydroxylations and catalyzed testosterone 6 beta-hydroxylation. It is, therefore, suggested that although the spectral and immunochemical properties and the N-terminal amino acid sequence of P-450-MK1 were similar to those of P-450-male, the physiological functions of P-450-MK1 may be somewhat different from those of P-450-male. Comparison of the physico-chemical properties of P-450-MK1 with those of P-450-D1 and P-450-HM2, w...Continue Reading

References

Apr 1, 1978·Analytical Biochemistry·A Aitio
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S T OkinoR H Tukey
Jan 1, 1987·Annual Review of Biochemistry·D W Nebert, F J Gonzalez
Dec 28, 1984·Biochemical and Biophysical Research Communications·S OhmoriH Kitagawa

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Citations

May 7, 1992·Biochimica Et Biophysica Acta·M KomoriT Kamataki
Dec 21, 2006·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y UnoH Fujino
Jun 24, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R J WeaverM D Burke

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