Comparative study of demineralized collagen degradation determined by hydroxyproline assay and microscopic depth measurement

Journal of Dentistry
Md Sofiqul IslamJunji Tagami

Abstract

Quantification of collagen degradation is an important parameter to evaluate dentin caries progression or the efficacy of caries prevention aid. The aim of this study was to validate the simple light microscopic technique (LM) to evaluate collagen degradation by comparing with hydroxyproline assay technique (HPN). Bovine root dentin blocks were embedded in acrylic resin, polished and covered with nail varnish except a 1.5 × 2.5mm window. The specimens were demineralized in acetate buffer (pH 4.3) for 3 days to create incipient lesions and were exposed to collagenase enzyme for 6, 9 and 16 h. The specimens were sectioned into thin sections (200-220 μm) to measure the degraded depth of collagen matrix by LM. The enzyme solutions were allocated to HPN assay using the simplified chloramines-T method. Correlation between LM and HPN was evaluated by Pearson correlation analysis. Anti-collagen degradation efficacy of 0.12% chlorhexidine (CHX) was evaluated by LM. The depths of the degraded collagen and amount of hydroxyproline in 3 exposure periods were 27.8 ± 3.8 μm and 28.7 ± 4.2 μg for 6h, 48.1 ± 8.6 μm and 45.3 ± 6.1 μg for 9h, and 74.2 ± 9.7 μm and 71.3 ± 8.0 μg for 16 h, respectively. A significantly positive correlation (r=0.94...Continue Reading

Citations

May 16, 2018·Experimental Biology and Medicine·Agnes B MeirelesLibardo A González-Torres
Dec 11, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Luyi ZhaoXinhua Gu

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