Comparative study of human red blood cell analysis with three different field-flow fractionation systems

Journal of Chromatography. B, Biomedical Applications
R ParsonsK D Caldwell

Abstract

An extensive multi-laboratory study was conducted to compare three different field-flow fractionation (FFF) systems for use in the analysis of human erythrocytes. The object of this study was to determine the relationship between the FFF elution properties for each system and the traditional hematological blood cell parameters. One centrifugal system (Utah) and two gravitational systems (Paris and Abbott) were compared. In order to analyze erythrocyte populations with a broad range of hematological indices, blood samples were collected from individuals heterozygous for sickle cell anemia (A/S) and also from normal controls (A/A), and these were analyzed at each site. Identical samples were analyzed by the Abbott and Utah sites. With all three systems, blood samples from each category produced narrow, overlapping distributions of FFF retention ratios, with the Abbott and Utah systems showing slight elevations in the mean retention ratios for the sickle cell samples. Blood cell elution peak characteristics were compared with standard hematological parameters for each of the FFF systems, and negative correlations were consistently found between mean corpuscular volume (MCV) and retention ratios. Positive correlations were found be...Continue Reading

References

Jul 17, 1991·Journal of Chromatography·P J CardotM Martin

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Citations

Jul 24, 2008·Analytical and Bioanalytical Chemistry·Barbara RodaAndrea Bontadini
Oct 1, 2008·Journal of Pharmaceutical Sciences·Hanns-Christian MahlerSylvia Kiese
Sep 8, 2009·Journal of Chromatography. a·G Bégaud-GrimaudP J P Cardot
Sep 23, 1997·Analytical Biochemistry·J M MetreauS Loric
Oct 17, 2002·Analytical Chemistry·Pierluigi ReschiglianGiovanna Cenacchi

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