Comparative study of methylene blue and erythrosine dyes employed in photodynamic therapy for inactivation of planktonic and biofilm-cultivated Aggregatibacter actinomycetemcomitans

Photomedicine and Laser Surgery
Rosangela de Carvalho GoulartPietro Ciancaglini

Abstract

The proposal of this work was to test the efficacy of photodynamic therapy (PDT) by using methylene blue (MB) and erythrosine (ERY) to inactivate Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), the main pathogen in aggressive periodontitis. A. actinomycetemcomitans was cultivated in planktonic cultures and biofilm by using Tryptic Soy Broth medium. The sensibility (dark toxicity) to MB and ERY was determined, and its ideal concentration for PDT protocols was established. An odontologic resin photopolymerizer was used as the light source. The bacterial viability was determined by CFU (planktonic cultures) and microscopic observation (biofilms). The results show that ERY is more efficient at killing bacterial cells of A. actinomycetemcomitans in planktonic (75%) and biofilm (77%) culture compared with MB (50% and 54%, respectively). PDT using MB or ERY as a photosensitizing agent and odontologic resin photopolymerizer as a light source could be an efficient option for pocket decontamination in aggressive periodontal disease.

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Citations

Sep 26, 2013·Current Opinion in Pharmacology·Rui YinMichael R Hamblin
Sep 20, 2015·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·J F TahmassebiS R Wood
Mar 18, 2011·Lasers in Surgery and Medicine·Stefan KranzBernd W Sigusch
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Dec 3, 2014·Photodiagnosis and Photodynamic Therapy·Letícia Heineck AlvarengaEduardo Saba-Chujfi
May 1, 2015·Current Microbiology·Natália Norika YassunakaJane Martha Graton Mikcha
May 18, 2016·Photodiagnosis and Photodynamic Therapy·Hadda Lyzandra Austríaco LeiteMarco Aurélio Paschoal
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Oct 23, 2018·Photomedicine and Laser Surgery·Iasmym Queiroz RomãoMarco Aurelio Benini Paschoal
Jun 12, 2012·Journal of Periodontology·Samira SalmeronEuloir Passanezi
Jul 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tianshou ZhangYanmin Zhou
Mar 13, 2021·Photodiagnosis and Photodynamic Therapy·Dongqing WangFengqiu Zhang
Jun 18, 2017·The Journal of Contemporary Dental Practice·Zahed MohammadiJun-Ichiro Kinoshita

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Methods Mentioned

BETA
irradiating

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