Comparative study of nuclear and cytoplasmic glycogen isolated from mutant HD33 ascites cells

Journal of Cellular Biochemistry
M KopunC R Krisman

Abstract

Mutant cells of the HD33 subline of the Ehrlich-Lettré ascites tumor synthesize and store glycogen mainly intranuclearly, when growing in vivo, and exclusively in the cytoplasm, when permanently cultivated as a suspension cell strain. To investigate whether there exist differences between glycogen of nuclear and cytoplasmic origin, the ultrastructure and the biophysical and biochemical properties of glycogen from in vivo and in vitro grown HD33 ascites cells were compared. Pronounced heterogeneity and differences in glycogen particle ultrastructure were evident in situ and after isolation of the native, high-molecular polysaccharide. Nuclear glycogen contains a fraction of heavier molecules (up to 2 X 10(9)) and larger particles (up to 340 nm) which could not be found in the cytoplasmic preparations, which contained only particles smaller than 140 nm. The subparticles of beta-type are similar in both nuclear and cytoplasmic glycogen. The absorption spectra and glucose analysis after degradation with phosphorylase and debranching enzyme indicate that nuclear glycogen has a higher degree of branching, associated with a decrease in the average chain length between the branching points, and shorter external polyglucosidic chains th...Continue Reading

References

Jun 1, 1975·Journal of Molecular and Cellular Cardiology·V J FerransW C Roberts
Jan 1, 1976·Journal of Ultrastructure Research·H P ZimmermannC Granzow
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Feb 1, 1966·Archives of Biochemistry and Biophysics·J MordohL F Leloir
Sep 1, 1967·Archives of Biochemistry and Biophysics·A J ParodiJ Mordoh
Oct 18, 1982·FEBS Letters·M KopunC Granzow
Jan 1, 1981·Journal of Cancer Research and Clinical Oncology·C Granzow
Apr 1, 1957·Archives of Biochemistry and Biophysics·R MONTGOMERY
Jan 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·J MORDOHC R KRISMAN
Jul 1, 1962·Analytical Biochemistry·C R KRISMAN
Sep 9, 1950·Nature·W E TREVELYANJ S HARRISON

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Citations

Nov 26, 2010·Science China. Life Sciences·Jia LiNing Li

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