Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
D MartinE Gómez-Lucía

Abstract

The choice of a diagnostic method depends on the characteristics of the herd to be analysed. Two herds with different prevalences of enzootic bovine leukaemia were chosen to study the concordance between agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) methods. PCR, an increasingly used virological method, was performed with four sets of primers, amplifying different genomic regions (env, pol and tax), from DNA extracted either from peripheral blood monocytes (PBMCs) or milk leucocytes. The highest percentage of positive animals was obtained using PCR performed with DNA extracted from PBMCs using primers which amplified either env or pol, followed by PCR using PBMCs and primers which hybridized with tax, then ELISA using serum and finally AGID. The results of PCR were more consistent with PBMCs than when milk leucocytes were used.

References

Feb 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A Ballagi-PordányS Belák
Feb 1, 1991·The Journal of General Virology·M L StottJ L Stott
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N SagataY Ikawa
Jan 1, 1984·Current Topics in Microbiology and Immunology·J GhysdaelA Burny
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·L WillemsR Kettmann
Jul 1, 1994·Journal of Virology·I SchwartzD Levy
Feb 1, 1993·Journal of Virology·L WillemsR Kettmann
Jan 1, 1993·Journal of Virological Methods·H PoonB Jefferson
Jun 1, 1996·Molecular and Cellular Probes·A Ballagi-Pordány, S Belák
Dec 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H FechnerD Beier
Mar 1, 1997·The Veterinary Clinics of North America. Food Animal Practice·J F Evermann, M K Jackson
Mar 1, 1997·The Veterinary Clinics of North America. Food Animal Practice·S G Hopkins, R F DiGiacomo
Jan 22, 1998·Veterinary Immunology and Immunopathology·A DoménechE Gómez-Lucía
Nov 26, 1999·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·G Dolz, E Moreno
Jan 21, 2000·The Journal of General Virology·A DoménechE Gómez-Lucía
Jul 14, 2000·Journal of Virological Methods·L LlamesE Gomez-Lucia
Sep 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M Reichert, J Stec

❮ Previous
Next ❯

Citations

Jul 20, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L LlamesJ Goyache
Jun 8, 2012·The Journal of Veterinary Medical Science·Michihito TagawaHisashi Inokuma
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gustavo E MontiMart C M de Jong
Nov 4, 2017·Virology Journal·Meripet PolatYoko Aida
Jul 10, 2017·Archives of Virology·Nayely HeineckeHugo Ramírez
Jul 19, 2013·Journal of Immunoassay & Immunochemistry·Clarissa C de CastroSilvia de O Hübner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.