PMID: 8961156Nov 1, 1996Paper

Comparative study of separation of non-encapsulated drug from unilamellar liposomes by various methods

The Journal of Pharmacy and Pharmacology
S R DipaliG V Betageri

Abstract

The purpose of this study was to compare the various methods available to separate non-encapsulated drug from large unilamellar liposomes (LUV). Multilamellar liposomes (MLV) were prepared by thin film hydration using distearoylphosphatidylcholine:cholesterol (2:1 molar ratio). MLVs were passed through a 0.2 micron polycarbonate membrane using an extruder to prepare LUVs. Particle size of liposome preparations was characterized using a submicron particle-size analyser. The non-encapsulated drug was separated by: filtering through Centrifree tubes; passing through gel (Sepharose-4B and Sephadex G-25M); passing through minicolumn; ficoll density gradient; protamine aggregation; or dialysis. The dialysis method was found to be unsuitable for separation of non-encapsulated drug due to equilibration of encapsulated drug as the free drug was dialyzed. The upper limit for lipid concentration was 5 mg mL-1 using the Centrifree method. Separation using gel chromatography led to dilution of liposome preparation. Minicolumn and density gradient techniques did not lead to sample dilution, however the minicolumn method was tedious. The time required for separation of liposomes by protamine aggregation was longer for neutral liposomes. Thus ...Continue Reading

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Citations

Jan 3, 2012·The Journal of Membrane Biology·Carla MatosPaulo Lobão
Mar 7, 2003·Journal of Inorganic Biochemistry·Nicholas R BlandfordRoy E Wuthier
Apr 14, 2012·ISRN Pharmaceutics·Krishna MuppidiGuru Betageri
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