PMID: 16521587Mar 9, 2006Paper

Comparative study of superoxide dismutase activity assays in Crocus sativus L. corms

Prikladnaia biokhimiia i mikrobiologiia
E Keyhani, J Keyhani

Abstract

Superoxide dismutase catalyzes the breakdown of the superoxide radical anion and provides the first line of defense against oxygen toxicity. Its vital importance has made it the subject of numerous investigations. Several assays have been proposed for the detection and quantitation of superoxide dismutase activity, but their use has remained controversial and no comparative studies have been reported. In this investigation, three commonly used methods were compared for the measurement of superoxide dismutase activity in Crocus sativus L. corm extract. The methods, based on a competition between the enzyme itself and another superoxide scavenger, involved respectively cytochrome c reduction, nitro blue tetrazolium reduction, and pyrogallol autoxidation. Because of its accuracy, reproducibility, simplicity and cost benefit, the latter method was the most appropriate.

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Citations

May 11, 2013·Analytical and Bioanalytical Chemistry·Claudia Swart
Dec 7, 2010·Journal of Medicinal Food·Mi-Young YoonHae-Ryong Park
Mar 7, 2014·Toxicon : Official Journal of the International Society on Toxinology·L J MattosR M Soares
Oct 16, 2012·Ecotoxicology and Environmental Safety·Sk Tofajjen HossainSamir Kumar Mukherjee
Dec 27, 2011·Plant Science : an International Journal of Experimental Plant Biology·Adelaide TurchiMilvia Luisa Racchi
Mar 8, 2007·Annals of the New York Academy of Sciences·Ezzatollah KeyhaniMahnaz Hadizadeh
May 29, 2013·Asian Pacific Journal of Tropical Medicine·Dolly JaiswalGeeta Watal
Jan 22, 2013·Journal of Bacteriology·Patima PermpoonpattanaSimon M Cutting

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