PMID: 11926993Apr 3, 2002Paper

Comparative study of the reaction mechanism of family 18 chitinases from plants and microbes

Journal of Biochemistry
Chiye SasakiTamo Fukamizo

Abstract

Hydrolytic mechanisms of family 18 chitinases from rice (Oryza sativa L.) and Bacillus circulans WL-12 were comparatively studied by a combination of HPLC analysis of the reaction products and theoretical calculation of reaction time-courses. All of the enzymes tested produced beta-anomers from chitin hexasaccharide [(GlcNAc)(6)], indicating that they catalyze the hydrolysis through a retaining mechanism. The rice chitinases hydrolyzed predominantly the fourth and fifth glycosidic linkages from the nonreducing end of (GlcNAc)(6), whereas B. circulans chitinase A1 hydrolyzed the second linkage from the nonreducing end. In addition, the Bacillus enzyme efficiently catalyzed transglycosylation, producing significant amounts of chitin oligomers larger than the initial substrate, but the rice chitinases did not. The time-courses of (GlcNAc)(6) degradation obtained by HPLC were analyzed by theoretical calculation, and the subsite structures of the rice chitinases were identified to be (-4)(-3)(-2)(-1)(+1)(+2). From the HPLC profile of the reaction products previously reported [Terwisscha van Scheltinga et al. (1995) Biochemistry 34, 15619-15623], family 18 chitinase from rubber tree (Hevea brasiliensis) was estimated to have the same...Continue Reading

Citations

Nov 9, 2006·Applied Microbiology and Biotechnology·Kenji MorimotoKazuo Sakka
Jan 18, 2011·Plant Molecular Biology·Takayuki OhnumaTamo Fukamizo
Mar 22, 2013·World Journal of Microbiology & Biotechnology·Soheila MatroodiMehdi Moradyar
Jul 2, 2009·Journal of Chemical Biology·Wipa SugintaHeino Prinz
Mar 30, 2004·Archives of Biochemistry and Biophysics·Wipa SugintaLinda A Fothergill-Gilmore
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Chomphunuch SongsiriritthigulWipa Suginta
Sep 8, 2011·Bioscience, Biotechnology, and Biochemistry·Tamo FukamizoSaori Takahashi
Apr 26, 2005·Bioscience, Biotechnology, and Biochemistry·Mitsuhiro UedaKazutaka Miyatake
Feb 23, 2013·Bioresource Technology·Katta Suma, Appa Rao Podile
Oct 18, 2008·Analytical Biochemistry·Karen Ghauharali-van der VlugtJohannes M F G Aerts
Aug 8, 2018·Plant Molecular Biology·Yiming FengTamo Fukamizo
Oct 10, 2006·Insect Biochemistry and Molecular Biology·Fernando A GentaClélia Ferreira
May 11, 2007·Biochimica Et Biophysica Acta·Wipa SugintaJisnuson Svasti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.