Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF

Journal of Proteome Research
Wells W WuRong-Fong Shen

Abstract

A comparative study on the three quantitative methods frequently used in proteomics, 2D DIGE (difference gel electrophoresis), cICAT (cleavable isotope-coded affinity tags) and iTRAQ (isobaric tags for relative and absolute quantification), was carried out. DIGE and cICAT are familiar techniques used in gel- and LC-based quantitative proteomics, respectively. iTRAQ is a new LC-based technique which is gradually gaining in popularity. A systematic comparison among these quantitative methods has not been reported. In this study, we conducted well-designed comparisons using a six-protein mixture, a reconstituted protein mixture (BSA spiked into human plasma devoid of six abundant proteins), and complex HCT-116 cell lysates as the samples. All three techniques yielded quantitative results with reasonable accuracy when the six-protein or the reconstituted protein mixture was used. In DIGE, accurate quantification was sometimes compromised due to comigration or partial comigration of proteins. The iTRAQ method is more susceptible to errors in precursor ion isolation, which could be manifested with increasing sample complexity. The quantification sensitivity of each method was estimated by the number of peptides detected for each prot...Continue Reading

References

Feb 6, 2002·Current Opinion in Chemical Biology·Kathryn S LilleyPaul Dupree
Feb 13, 2002·Electrophoresis·Michael FountoulakisLaura Suter
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wayne F Patton
Jan 21, 2003·Mass Spectrometry Reviews·Mahmoud Hamdan, Pier Giorgio Righetti
Feb 5, 2003·Current Opinion in Biotechnology·Michael B Goshe, Richard D Smith
Jul 16, 2004·Journal of Proteome Research·Samir Julka, Fred Regnier
Nov 10, 2004·Journal of Chromatography. a·Pier Giorgio RighettiMahmoud Hamdan
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Alexander Leitner, Wolfgang Lindner
Feb 1, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haleem J IssaqTimothy D Veenstra

❮ Previous
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Citations

Aug 11, 2007·Analytical and Bioanalytical Chemistry·N DelmotteC G Huber
Nov 7, 2007·Analytical and Bioanalytical Chemistry·Christina Rappel, Dirk Schaumlöffel
Mar 23, 2010·Analytical and Bioanalytical Chemistry·Susanne BomkeUwe Karst
Mar 30, 2011·Analytical and Bioanalytical Chemistry·Fabien XuerebJean-Marie Schmitter
Mar 5, 2009·Acta Neuropathologica·Maria ZellnerEllen Umlauf
Nov 13, 2008·Cytotechnology·Stephen Carberry, Sean Doyle
Dec 15, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Loyda M MeléndezDianedis Toro-Nieves
Apr 29, 2010·Current HIV/AIDS Reports·Adam BurgenerT Blake Ball
May 11, 2013·Journal of Biosciences·Riddhi DattaSharmila Chattopadhyay
Dec 24, 2009·Biological Procedures Online·Nakul MandalHenrik Vorum
Apr 7, 2009·Journal of the American Society for Mass Spectrometry·Jenny M ArmentaIulia M Lazar
Jun 30, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Nichole A ReisdorphCarolyn Broccardo
Oct 2, 2007·Journal of Proteome Research·Timothy J GriffinLeeAnn Higgins
Aug 4, 2010·Journal of Proteome Research·Jayme L WiederinPawel S Ciborowski
May 31, 2008·Journal of Proteome Research·Shama P MirzaMichael Olivier
Feb 26, 2013·Asian Journal of Andrology·Pierre BigotOlivier Cussenot
Jul 13, 2011·Nature Biotechnology·Martin Latterich, Jan E Schnitzer
Apr 5, 2007·Nature Protocols·Tal IsaacsonJocelyn K C Rose
Apr 5, 2007·Nature Protocols·Odile CarretteDenis F Hochstrasser
Apr 13, 2010·Molecular & Cellular Proteomics : MCP·Natasha A KarpKathryn S Lilley
Feb 27, 2014·Biotechnology & Genetic Engineering Reviews·Mian Yahya MushtaqHye Kyong Kim
Apr 14, 2010·Zebrafish·Hendrian SukardiSiew Hong Lam
Jun 27, 2008·Briefings in Functional Genomics & Proteomics·Mu WangDawn P G Brown
Jun 11, 2010·Briefings in Bioinformatics·Pascal RoyNicolas Molinari
Mar 20, 2010·Brain : a Journal of Neurology·Manuel ComabellaXavier Montalban
Jul 3, 2009·Journal of the Royal Society, Interface·Laura E McNamaraRichard Burchmore
Dec 7, 2007·The Plant Cell·Jay J Thelen, Scott C Peck
Jan 30, 2010·Journal of Applied Physiology·Adam G EverttsBenjamin A Garcia
May 15, 2010·International Journal of Biomedical Imaging·Kimberly F Sellers, Jeffrey C Miecznikowski
Mar 2, 2012·Journal of Nutrigenetics and Nutrigenomics·Isabel Bondia-PonsJ Alfredo Martínez
May 27, 2010·Malaria Journal·Sébastien BriolantBruno Pradines
Sep 26, 2007·Proteome Science·Jean-Philippe GagnéGuy G Poirier
Jul 1, 2011·Journal of Translational Medicine·Chuanyu SunQiang Ding
Nov 7, 2007·Biology Direct·Gelio AlvesYi-Kuo Yu
Apr 17, 2008·Saline Systems·Jagroop PandhalCatherine A Biggs

❮ Previous
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