Comparative Study of Toluidine Blue O and Methylene Blue Binding to Lysozyme and Their Inhibitory Effects on Protein Aggregation

ACS Omega
Baishakhi SahaGopinatha Suresh Kumar

Abstract

A comparative binding interaction of toluidine blue O (TBO) and methylene blue (MB) with lysozyme was investigated by multifaceted biophysical approaches as well as from the aspects of in silico biophysics. The bindings were static, and it occurred via ground-state complex formation as confirmed from time-resolved fluorescence experiments. From steady-state fluorescence and anisotropy, binding constants were calculated, and it was found that TBO binds more effectively than MB. Synchronous fluorescence spectra revealed that binding of dyes to lysozyme causes polarity changes around the tryptophan (Trp) moiety, most likely at Trp 62 and 63. Calorimetric titration also depicts the higher binding affinity of TBO over MB, and the interactions were exothermic and entropy-driven. In silico studies revealed the potential binding pockets in lysozyme and the participation of residues Trp 62 and 63 in ligand binding. Furthermore, calculations of thermodynamic parameters from the theoretical docking studies were in compliance with experimental observations. Moreover, an inhibitory effect of these dyes to lysozyme fibrillogenesis was examined, and the morphology of the formed fibril was scanned by atomic force microscopy imaging. TBO was ob...Continue Reading

References

Oct 20, 1989·Journal of Molecular Biology·J H HaM T Record
Oct 1, 1965·American Journal of Surgery·D P SheddS Bahn
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T ImotoF Tanaka
Dec 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·E M Tuite, J M Kelly
Jul 27, 2000·Journal of Clinical Pathology·A M van den Besselaar, A C Moor
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·D C MalinsM A Vinson
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jean-François TremblayRobert Bissonnette
Aug 28, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Massimo Stefani, Christopher M Dobson
Oct 30, 2003·Critical Reviews in Microbiology·Barbara Masschalck, Chris W Michiels
Feb 14, 2004·Redox Report : Communications in Free Radical Research·R Heiner SchirmerBocar Kouyaté
Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Zhenyu GuHai-Meng Zhou
May 12, 2004·Biochimica Et Biophysica Acta·Vladimir N Uversky, Anthony L Fink
Jul 9, 2004·Journal of Molecular Biology·Erica FrareAngelo Fontana
Nov 16, 2004·Journal of Photochemistry and Photobiology. B, Biology·Qing HuangQiang Xue
Feb 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Joris T MeijerJan C M van Hest
Jun 10, 2008·Bioscience, Biotechnology, and Biochemistry·Akihito HaradaAkio Kato
Oct 16, 2008·Current Protein & Peptide Science·Vladimir N Uversky
Oct 27, 2009·Colloids and Surfaces. B, Biointerfaces·Jing LiuBozhong Mu
Nov 6, 2009·Physical Chemistry Chemical Physics : PCCP·Naima NarbandIvan P Parkin
Mar 23, 2011·The Journal of Physical Chemistry. B·Mily BhattacharyaSamrat Mukhopadhyay
Jun 15, 2011·Angewandte Chemie·Maria F MossutoXavier Salvatella
Aug 26, 2011·The Journal of Physical Chemistry. B·Anna I SulatskayaKonstantin K Turoverov
Jan 3, 2012·Archives of Oral Biology·Simone Furgeri Godinho VilelaAntonio Olavo Cardoso Jorge
Mar 14, 2012·The Journal of Biological Chemistry·Lubert Stryer
Feb 26, 2014·Journal of Photochemistry and Photobiology. B, Biology·Asma Yasmeen KhanGopinatha Suresh Kumar
Apr 20, 2014·Journal of Hazardous Materials·Anirban Basu, Gopinatha Suresh Kumar

❮ Previous
Next ❯

Citations

Aug 31, 2020·PeerJ·Greta MusteikyteVytautas Smirnovas
Sep 3, 2020·Acta Crystallographica. Section D, Structural Biology·Marina Plaza-GarridoAna Camara-Artigas
Nov 7, 2020·International Journal of Biological Macromolecules·Mengna WangXinwu Ba
Sep 7, 2019·ACS Applied Materials & Interfaces·Ashleigh AndersonJames Davis

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism
atomic force microscopy
Fluorescence
fluorescence spectroscopy
AFM
column chromatography

Software Mentioned

FTSite
Chimera
EADock DSS
Avogadro
JASCO
Origin
protScale
PicoView
SwissDock
lyz

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.