Abstract
Several anaphylatoxic substances (human C3a, guinea pig C3a, human C4a, guinea pig C5a, and a synthetic C3a-related hexapeptide) were compared with regard to their ability to induce secretion of [3H] serotonin from guinea pig platelets. Functional identity of the C3a preparations, C4a, and the hexapeptide was demonstrated by the phenomenon of crossed desensitization. Whereas C3a of human and guinea pig origin proved to be qualitatively and quantitatively identical, C4a expressed only 3% of the activity of the C3 fragments on a molar basis. Investigations with goat anti-guinea pig C3a demonstrate that human and guinea pig C3a possess one antigenic determinant in common; however, this determinant is not the C-terminal amino acid sequence. Addition of the anaphylatoxins with low doses of thrombin led to a potentiation of [3H] serotonin release from the platelets. Under these conditions C3a concentrations of 1.5 X 10(-10) mumol/liter (65 pg of C3a) could be detected. Thus the platelet system represents the most sensitive in vitro assay known for evaluation of biological activity of the C3a anaphylatoxins.
References
Jun 1, 1978·Inflammation·R O Webster, P M Henson
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T E Hugli, B W Erickson
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J P GorskiH J Müller-Eberhard
Nov 14, 1978·Biochemistry·J W JacobsR A Bradshaw
Jan 1, 1978·Contemporary Topics in Molecular Immunology·T E Hugli
Jan 1, 1978·Scandinavian Journal of Immunology·S BeckerD Bitter-Suermann
Jan 1, 1978·Scandinavian Journal of Immunology·S BeckerD Bitter-Suermann
Jun 1, 1976·European Journal of Immunology·C BentleyV Brade
May 1, 1973·The Journal of Experimental Medicine·E H Vallota, H J Müller-Eberhard
Feb 1, 1968·The Journal of Experimental Medicine·C G Cochrane, H J Müller-Eberhard
Oct 1, 1980·Molecular Immunology·D Bitter-SuermannR Andreatta
May 1, 1964·The Journal of Clinical Investigation·R H ASTER, J H JANDL
Citations
Sep 1, 1981·Immunopharmacology·S MeuerD Bitter-Suermann
Jan 27, 2000·Immunopharmacology·P H PfeiferT E Hugli
Aug 1, 1983·The Journal of Experimental Medicine·M J Polley, R L Nachman
Sep 18, 2007·Immunologic Research·Pieter-Jan Haas, Jos van Strijp
Nov 1, 1989·Kidney International·R J JohnsonW G Couser
Apr 3, 2012·Immunobiology·Philipp SchoengrafAnita Ignatius
Mar 25, 2005·Vox Sanguinis·C P TurnerR Cardigan
May 1, 1990·Cellular Immunology·H MontzO Götze
Sep 1, 1991·Immunology Today·P J Sims, T Wiedmer
Nov 1, 1993·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·M Haeger
Dec 1, 1985·Scandinavian Journal of Immunology·I von ZabernW Vogt
Mar 29, 1991·Biochemical and Biophysical Research Communications·Y FukuokaT Tachibana
Apr 16, 1991·Biochemistry·J A EmberT E Hugli
Jan 12, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Stefan P KastlJohann Wojta
Feb 5, 2021·Research and Practice in Thrombosis and Haemostasis·Xu HanMarvin T Nieman
Apr 29, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D J BirminghamA A Creasey