Comparative Study on In Vitro Culture of Mouse Bone Marrow Mesenchymal Stem Cells

Stem Cells International
Yuxin HuYuanyuan Xu

Abstract

In vitro culture of mesenchymal stem cells (MSCs) from mouse bone marrow (BM) has been hampered because of the low yield of MSCs during isolation and the contamination of hematopoietic cells during expansion. The lack of specific mouse BM-MSC markers increases the difficulty. Several techniques have been reported to improve the purity and in vitro growth of mouse BM-MSCs. However, systematic report on comparison of characteristics in primary BM-MSCs between different culture conditions is rare. Here, we studied the effects of oxygen concentrations and initial medium replacement intervals, along with cell passages, on mouse BM-MSCs isolated with differential adhesion method. BM-MSCs exhibited elevated proliferative and clonogenic abilities in 5% oxygen compared with 10% and 21% oxygen, as well as a better expression of the MSC marker Sca-1. Adipogenic and osteogenetic differentiation of BM-MSCs can be observed in both 21% and 5% oxygen. Adipogenic differentiation appeared stronger under normoxia conditions. BM-MSCs showed increased proliferative capacity and adipogenic/osteogenetic differentiation potential when initial medium replacement interval was 4 days compared with 1 day. As passage number increased, cells were more MSC-l...Continue Reading

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Citations

Feb 15, 2020·International Journal of Molecular Sciences·Lilla HámoriGergely Szakács
Sep 19, 2019·Biotechnology and Bioengineering·Mikhael Hadida, David Marchat
Jan 19, 2020·Tissue Engineering. Part B, Reviews·Steven James AveryRachel Jane Waddington
Dec 6, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Simran KaurGarima Khare
May 27, 2021·Hematology, Transfusion and Cell Therapy·Mariana Ferreira PissarraMariana Lazarini

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism

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