Comparative study on metabolite level in tissue-specific human mesenchymal stem cells by an ultra-performance liquid chromatography quadrupole time of flight mass spectrometry

Analytica Chimica Acta
Seul Ji LeeSung Won Kwon

Abstract

Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands. Using an optimized metabolomics technique, we verified that human MSCs exhibit differences in the endogenous metabolite level depending on their source material, while the multivariate analysis showed that 5 lysophosphatidylcholines and 3 lysophosphatidylethanolamines can serve as markers for the discrimination between MSC sources and may be related to differences in their differentiation capacity. These results may significantly contribute to further mechanistic studies on the MSCs and provide novel insights into the properties and optimal usage of MSCs from different tissues.

Citations

Nov 6, 2018·Current Stem Cell Research & Therapy·Dilcele Silva Moreira DziedzicKatherine Athayde Teixeira de Carvalho
Nov 24, 2019·Seminars in Cell & Developmental Biology·Jason S RockelSowmya Viswanathan
Jun 17, 2021·Stem Cell Reviews and Reports·Daniela S C BispoAna M Gil
Jul 13, 2021·World Journal of Stem Cells·Aline QueirozMarinella Holzhausen
Aug 24, 2021·Current Opinion in Biotechnology·Maria J SebastiãoPaula M Alves

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