Comparative study on photosynthetic activity of chloroplasts in acid and alkaline zones of Chara corallina

Bioelectrochemistry
Alexander A BulychevStefan C Müller

Abstract

A novel experimental approach has been applied to investigate the relationship between pH banding in Chara cells and photosynthetic activity of chloroplasts located in cell regions adjacent to acid and alkaline bands. The combination of pH microelectrode technique with pulse amplitude modulation (PAM) microfluorimetry enabled parallel measurements of longitudinal pH profiles and chlorophyll fluorescence yield in acid and alkaline zones of individual Chara cells. The scanning with a pH-microelectrode along the cell length revealed the light-dependent pH pattern, i.e., alternating acid and alkaline bands with pH differences as large as 2 - 3 pH units. In parallel, measurements of chlorophyll fluorescence yield under actinic light were performed using PAM microfluorometry. It was found that the effective photochemical yield of photosystem II is substantially higher in acid than in alkaline zones. The results clearly show that the banding pattern is not confined solely to the plasmalemma but is also exhibited in alternating photosynthetic performance of the underlying chloroplast layer. Apparently, the acid regions enriched with CO2 ensure sufficient flow of this substrate to the Calvin cycle reactions, thus promoting the photosynt...Continue Reading

Citations

Jul 31, 2012·Photosynthesis Research·John A Raven, Catriona L Hurd
Jun 6, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Natalia A KrupeninaUlrich Schreiber
Sep 11, 2008·Plant & Cell Physiology·Teruo Shimmen, Akiko Wakabayashi
Jun 11, 2011·Plant & Cell Physiology·Patric M SchmölzerIlse Foissner
May 14, 2014·Biochemistry. Biokhimii︠a︡·A A Bulychev, A V Komarova
Mar 19, 2014·Plant, Cell & Environment·Vladimir SukhovVladimir Vodeneev
Nov 29, 2011·Journal of Microscopy·I Foissner, G O Wasteneys
Jul 24, 2012·Trends in Plant Science·Alison R TaylorGlen L Wheeler
Jan 4, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alexey EreminAndrei B Rubin
Jan 23, 2015·Frontiers in Plant Science·Vladimir SukhovVladimir Vodeneev
Aug 2, 2019·Protoplasma·Alexey EreminIlse Foissner
Oct 2, 2002·Plant & Cell Physiology·Teruo Shimmen, Ako Yamamoto
Feb 11, 2020·Frontiers in Plant Science·Suyun ZhangBert van Duijn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.