PMID: 9533761Apr 9, 1998Paper

Comparative study on the induction of cytostasis and apoptosis by ICI 182,780 and tamoxifen in an estrogen receptor-negative ovarian cancer cell line

International Journal of Cancer. Journal International Du Cancer
A ErcoliS Mancuso

Abstract

We have compared the effects of a broad range of clinically relevant concentrations (0.1 to 10 microM) of the steroidal pure anti-estrogen ICI 182,780 and the non-steroidal partial anti-estrogen tamoxifen (TAM) on cell proliferation and induction of apoptosis in the estrogen receptor (ER)-negative ovarian carcinoma cell line A2780. Cell proliferation was assessed by evaluating the number of viable cells, changes in cell-cycle distribution and cell replication rate; while apoptosis induction was assessed by examining nuclear morphological changes associated with apoptotic death and DNA cleavage into 300 and 50 kbp units (large DNA fragmentation) and into 180 bp units (internucleosomal DNA fragmentation). We provide evidence that 0.1 to 10 microM ICI 182,780 and TAM significantly inhibit the growth of A2780 cells in a dose-dependent fashion. Cytokinetic analysis revealed that only 10 microM TAM caused a significant blockade in G1 and a diminished replication rate. Conversely, we show that 0.1 to 10 microM ICI 182,780 and TAM induce apoptosis in a dose-dependent fashion. The earliest recognizable apoptotic change induced by treating the cells with these 2 drugs was DNA cleavage into 300 and 50 kbp units. This started to be visible...Continue Reading

References

Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·T TaniguchiT Miyata
Jan 1, 1988·Journal of Steroid Biochemistry·A E Wakeling, J Bowler
Apr 1, 1995·Cancer Immunology, Immunotherapy : CII·K IwasakiB P Barna
May 16, 1995·International Journal of Cancer. Journal International Du Cancer·J W WilsonC Dive
Feb 1, 1995·Experimental Cell Research·L M Desjardins, J P MacManus
Feb 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·I A JaiyesimiG N Hortobagyi
May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
May 20, 1994·Cell·E Ruoslahti, J C Reed
Jan 1, 1993·Breast Cancer Research and Treatment·A E Wakeling
Mar 6, 1996·Journal of the National Cancer Institute·Y KangR R Perry
May 1, 1996·Genes & Development·L J Ko, C Prives
Nov 4, 1996·International Journal of Cancer. Journal International Du Cancer·R De VincenzoS Mancuso
Jan 1, 1995·Trends in Cell Biology·C D BortnerJ A Cidlowski
Mar 1, 1997·Trends in Cell Biology

❮ Previous
Next ❯

Citations

Oct 14, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xi-Ying WangHong-Chun Liu
Mar 30, 2007·Molecular Carcinogenesis·Lauren FishbeinMargaret R Wallace
Oct 27, 2004·Molecular and Cellular Endocrinology·Hanna Savolainen-PeltonenPekka Häyry
May 13, 2005·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Ndiya OgbaSamir Raychoudhury
Jun 28, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jaya Pamidimukkala, Meredith Hay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis