Comparative substrate specificity analysis of recombinant human cathepsin V and cathepsin L

Archives of Biochemistry and Biophysics
Luciano PuzerA K Carmona

Abstract

Cathepsins V and L have high identity and few structural differences. In this paper, we reported a comparative study of the hydrolytic activities of recombinant human cathepsins V and L using fluorescence resonance energy transfer peptides derived from Abz-KLRSSKQ-EDDnp (Abz = ortho-aminobenzoic acid and EDDnp = N-(2,4-dinitrophenyl)ethylenediamine). Five series of peptides were synthesized to map the S3 to S2' subsites. The cathepsin V subsites S1 and S3 present a broad specificity while cathepsin L has preference for positively charged residues. The S2 subsites of both enzymes require hydrophobic residues with preference for Phe and Leu. The S1' and S2' subsites of cathepsins V and L are less specific. Based on these data we designed substrates to explore the electrostatic potential differences of them. Finally, the kininogenase activities of these cathepsins were compared using synthetic human kininogen fragments. Cathepsin V preferentially released Lys-bradykinin while cathepsin L released bradykinin. This kininogenase activity by cathepsins V and L was also observed from human high and low molecular weight kininogens.

References

Feb 17, 1995·Journal of Molecular Biology·P J Berti, A C Storer
Aug 9, 1993·FEBS Letters·R MénardA C Storer
Aug 1, 1996·The Journal of Experimental Medicine·H HerwaldL Björck
Apr 1, 1997·Protein Science : a Publication of the Protein Society·C J LinneversD Brömme
Nov 5, 1997·The Journal of Biological Chemistry·E Del NeryL Juliano
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·L Debelle, A M Tamburro
Jun 13, 2000·Advances in Experimental Medicine and Biology·F BühlingD K Nägler
Aug 10, 2000·Analytical Biochemistry·J C Krupa, J S Mort
Oct 12, 2000·Biochemistry·J R SomozaM E McGrath
Dec 21, 2002·European Journal of Biochemistry·Claire DesmazesGilles Lalmanach
Jan 30, 2003·Acta Crystallographica. Section D, Biological Crystallography·Dusan Turk, Gregor Guncar
May 31, 2003·Nature Reviews. Immunology·Karen Honey, Alexander Y Rudensky
Jun 5, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Jian LiuGuo-Ping Shi
Jun 12, 2004·The Journal of Biological Chemistry·Yoshiyuki YasudaDieter Brömme

❮ Previous
Next ❯

Citations

Oct 7, 2005·Biological Chemistry·Luciano PuzerAdriana K Carmona
Nov 26, 2005·Biological Chemistry·Luciano PuzerAdriana K Carmona
Sep 16, 2006·Biological Chemistry·Martin LinkeDieter Brömme
Oct 26, 2011·Biochimica Et Biophysica Acta·Vito TurkDušan Turk
Mar 2, 2005·Biochemical and Biophysical Research Communications·Gopal DevanathanTraian Sulea
Jan 25, 2006·Insect Biochemistry and Molecular Biology·A R LopesW R Terra
Jul 22, 2008·Molecular and Biochemical Parasitology·Maria F LanfrancoJorge Arévalo
May 29, 2007·Archives of Biochemistry and Biophysics·Mohamed HassaneinRobert W Mason
Aug 26, 2014·Analytical Biochemistry·Monika LęgowskaAdam Lesner
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Adriana K CarmonaLuiz Juliano
Feb 1, 2005·Archives of Biochemistry and Biophysics·Luciano PuzerAdriana K Carmona
Oct 15, 2013·The Journal of Biological Chemistry·Xin DuDieter Brömme
Nov 15, 2018·Scientific Reports·Natalia V PovarovaKonstantin A Lukyanov
May 5, 2016·The Journal of Biological Chemistry·Helena ÖhrvikDennis J Thiele
Nov 24, 2017·Protein Science : a Publication of the Protein Society·Sam L IvryCharles S Craik
Mar 25, 2019·Biochemical Society Transactions·Walter R TerraClélia Ferreira
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hualin LiCatarina E Hioe
Nov 17, 2020·Frontiers in Molecular Biosciences·Irina Y FilippovaElena N Elpidina
Jan 5, 2008·Journal of Medicinal Chemistry·Jeremy P MallariR Kiplin Guy
Sep 5, 2009·ACS Nano·Violaine SéeRaphaël Lévy
Sep 27, 2018·Biochimica Et Biophysica Acta. General Subjects·Jorge E Hernández-GonzálezPedro A Valiente

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.