Comparative susceptibilities of insect cell lines to infection by the occlusion-body derived phenotype of baculoviruses

Journal of Invertebrate Pathology
Dwight E Lynn

Abstract

Twelve insect cell lines from six species were tested for susceptibility to baculovirus infection by occlusion-derived virus (ODV) phenotype through the use of a typical endpoint assay procedure. ODV from three nucleopolyhedroviruses were prepared by alkali treatment (sodium carbonate) of occlusion bodies (OBs) and the virus preparations were titered on various cell lines. More than a four-log difference was realized for each of theses viruses between the various cell lines. The TN368 line from Trichoplusia ni was only marginally susceptible to ODV from each virus, showing only 3-6 infectious units (IU) per million OBs while the gypsy moth line, LdEp was most susceptible, realizing more than 100,000 IU/million OBs. The other lines tested showed various levels of susceptibility between these two extremes and also varied between the three viruses tested. In additional tests, the ODV were treated with trypsin prior to application to the cells. With most cell lines, this treatment increased the infectivity of each virus by 2-10-fold. Exceptions to this trend included the gypsy moth LdEp line, on which the trypsinized ODV from two of the viruses were slightly less infectious than each virus without trypsin, and the TN-368 line, on w...Continue Reading

References

Jan 1, 1975·Intervirology·E M DoughertyC F Reichelderfer
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·E K EngelhardL E Volkman
Sep 1, 1993·Journal of Invertebrate Pathology·P M ZanottoJ E Maruniak
Jan 1, 1996·Cytotechnology·G W Blissard
May 30, 1998·In Vitro Cellular & Developmental Biology. Animal·J J Grasela, A H McIntosh
Oct 24, 1998·Journal of Invertebrate Pathology·Dwight E Lynn, M Shapiro
Aug 31, 1999·Archives of Insect Biochemistry and Physiology·B Oppert
Mar 12, 2004·Annual Review of Entomology·F Moscardi
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Dwight E LynnH Oberlander

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Metazoa
Host-Parasite Interactions
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