Comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi

International Journal of Peptide and Protein Research
A S WaliV V Modi

Abstract

Temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. Temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the Arrhenius plots were linear between 5 degrees and 50 degrees. The energies of activation ranged from 2.1 Kcal/mol for the enzyme from Sporotrichum thermophile to 9.1 Kcal/mol for that from Penicillium duponti. Heat inactivation kinetics at 50 degrees revealed heat lability of the enzyme from most of the thermophilic fungi. The t1/2's (min) were less than 10 for the enzymes from P. duponti, S. thermophile and Thermoascus aurantiacus; less than 30 for those from Chaetomium thermophile var. coprophile, H. lanuginosa and C. thermophile var. dissitum; and greater than 30 for those from Mucor pusillus and H. insolens. Salts of Na+, K+ and NH4+, and citrate protected the enzymes from H. lanuginosa, C. thermophile var. dissitum and M. pusillus against heat inactivation.

References

Jan 1, 1976·Experientia. Supplementum·J H Biffen, R A Williams
Feb 3, 1975·Biochemical and Biophysical Research Communications·J W CrabbR E Amelunxen
Sep 19, 1975·Biochimica Et Biophysica Acta·P Komaratat, M Kates
Jun 18, 1974·Biochimica Et Biophysica Acta·A HachimoriY Noso
Aug 14, 1973·Biochemistry·G N Sando, P C Hogenkamp
Dec 1, 1973·Journal of Bacteriology·M W Griffiths, T K Sundaram
Jun 1, 1969·Canadian Journal of Microbiology·J L SumnerH C Evans
Mar 1, 1965·Archives of Biochemistry and Biophysics·K D MUNKRES, F M RICHARDS
Apr 1, 1952·Archives of Biochemistry and Biophysics·C MARSH, W MILITZER
Jun 1, 1972·Annals of the New York Academy of Sciences·G SzaboS Krasne

❮ Previous
Next ❯

Citations

Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·R MaheshwariM K Bhat
Jan 19, 2016·Critical Reviews in Microbiology·Bijender SinghTulasi Satyanarayana
May 26, 2017·Evolution; International Journal of Organic Evolution·Scott R Miller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.