Comparative testicular transcriptome of wild type and globozoospermic Dpy19l2 knock out mice

Basic and Clinical Andrology
Thomas KaraouzènePierre F Ray

Abstract

Globozoospermia is a male infertility phenotype characterized by the presence in the ejaculate of near 100% acrosomeless round-headed spermatozoa with normal chromosomal content. Following intracytoplasmic sperm injection (ICSI) these spermatozoa give a poor fertilization rate and embryonic development. We showed previously that most patients have a 200 kb homozygous deletion, which includes DPY19L2 whole coding sequence. Furthermore we showed that the DPY19L2 protein is located in the inner nuclear membrane of spermatids during spermiogenesis and that it is necessary to anchor the acrosome to the nucleus thus performing a function similar to that realized by Sun proteins within the LINC-complex (Linker of Nucleoskeleton and Cytoskeleton). SUN1 was described to be necessary for gametogenesis and was shown to interact with the telomeres. It is therefore possible that Dpy19l2 could also interact, directly or indirectly, with the DNA and modulate gene expression during spermatogenesis. In this study, we compared the transcriptome of testes from Dpy19l2 knock out and wild type mice in order to identify a potential deregulation of transcripts that could explain the poor fertilization potential of Dpy19l2 mutated spermatozoa. RNA was...Continue Reading

References

Dec 1, 1973·Journal of Reproduction and Fertility·A F HolsteinC G Schirren
Mar 30, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K SwannF A Lai
Sep 25, 2004·Journal of Cellular Physiology·Scott B HerrickRichard A Cardullo
Feb 11, 2005·Archives of Histology and Cytology·Abraham L Kierszenbaum, Laura L Tres
Aug 24, 2006·Nature Reviews. Molecular Cell Biology·Yonatan B TzurYosef Gruenbaum
Sep 30, 2006·Human Reproduction Update·A H D M DamJ A M Kremer
Sep 25, 2010·Journal of Andrology·Anika H DamJan A Kremer
Jan 15, 2011·Reproductive Biomedicine Online·A PerrinF Morel
Mar 18, 2011·Nature·Timo StrünkerU Benjamin Kaupp
Oct 21, 2011·Physiological Reviews·Alberto DarszonClaudia L Treviño
Feb 10, 2012·Spermatogenesis·Abraham L KierszenbaumLaura L Tres
Apr 19, 2012·Human Reproduction·Cristian Alvarez SedóHéctor E Chemes
May 11, 2012·Andrologia·A PerrinM De Braekeleer
May 26, 2012·Human Reproduction·Charles CouttonPierre F Ray
Dec 12, 2012·Cell Calcium·Karl Swann, F Anthony Lai
Feb 5, 2013·The International Journal of Biochemistry & Cell Biology·Subir K NagdasSamir Raychoudhury

❮ Previous
Next ❯

Citations

Nov 14, 2020·Best Practice & Research. Clinical Endocrinology & Metabolism·Julie BeuroisCharles Coutton

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
PCR
PCRs
electrophoresis
genotyping
chip
chips

Software Mentioned

Expression Console
PANTHER Gene List Analysis
PANTHER

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.