PMID: 39412May 1, 1979

Comparative tests of various liquid media for the preenrichment of salmonellae from milk powder (author's transl)

Zentralblatt Für Bakteriologie. 1. Abt. Originale B, Hygiene, Krankenhaushygiene, Betriebshygiene, Präventive Medizin
H E Müller

Abstract

In applying S. mendoza the following four liquid media were tested as to whether they can be used as enrichment broths for salmonellae in milk powder: phosphate buffered water, correspondingly buffered peptone broth, tetrathionate and selenite lactose broth. The yield of salmonellae in these tests is largely independent of the starting pH value of the prepared milk suspension which was varied between 5.8 and 8.2 (Fig. 1). In water the pH dropped within 24 hours (Fig. 2), in peptone and tetrathionate broth within 48 hours down to figures between 4.1 and 4.4 (Fig. 3 and 4), in selenite broth final figures of pH 5.7 were not attained before the lapse of 6 days (Fig. 5). The absolutely highest germ figures were observed in selenite broth. Quite in general the bacteria figure maximum was not attained until the 3rd--5th day, whereupon the germ figures dropped again. In peptone broth the introduced salmonellae were recovered in 73% of all cases after the lapse of 24 hours. Also in tetrathionate the success quota was about 73%, but part of it (8%) did not increase until a lapse of more than 72 hours incubation time, although the pH had already become strongly acid some days previously. In water the introduced salmonellae were traced on...Continue Reading

Related Concepts

Bos indicus
Food Microbiology
Hydrogen-Ion Concentration
Peptones
Salmonella
Selenium
Tetrathionic Acid, Disodium Salt, Dihydrate

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