Comparative transcript analyses of the ovule, microspore, and mature pollen in Brassica napus.

Plant Molecular Biology
Carrie A WhittleJoan E Krochko

Abstract

Transcriptome data for plant reproductive organs/cells currently is very limited as compared to sporophytic tissues. Here, we constructed cDNA libraries and obtained ESTs for Brassica napus pollen (4,864 ESTs), microspores (i.e., early stage pollen development; 6,539 ESTs) and ovules (10,468 ESTs). Clustering and assembly of the 21,871 ESTs yielded a total of 10,782 unigenes, with 3,362 contigs and 7,420 singletons. The pollen transcriptome contained high levels of polygalacturonases and pectinesterases, which are involved in cell wall synthesis and expansion, and very few transcription factors or transcripts related to protein synthesis. The set of genes expressed in mature pollen showed little overlap with genes expressed in ovules or in microspores, suggesting in the latter case that a marked differentiation had occurred from the early microspore stages through to pollen development. Remarkably, the microspores and ovules exhibited a high number of co-expressed genes (N = 1,283) and very similar EST functional profiles, including high transcript numbers for transcriptional and translational processing genes, protein modification genes and unannotated genes. In addition, examination of expression values for genes co-expressed...Continue Reading

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Citations

Jul 16, 2013·Plant Reproduction·Mercedes SorianoKim Boutilier
Aug 14, 2015·International Journal of Molecular Sciences·Chunqing LiuTingdong Fu
Mar 31, 2012·The Plant Journal : for Cell and Molecular Biology·Samik Bhattacharya, Ian T Baldwin
Jun 9, 2016·Plant Reproduction·Luca AmbrosinoMaria Luisa Chiusano
Jun 9, 2016·Plant Reproduction·Palak ChaturvediWolfram Weckwerth
Dec 29, 2020·Plant Communications·Felix E G BeaudryStephen I Wright
Apr 6, 2021·The New Phytologist·Palak ChaturvediDavid Honys

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