Comparative transcriptional profiling of Bacillus cereus sensu lato strains during growth in CO2-bicarbonate and aerobic atmospheres

PloS One
Karla D PassalacquaNicholas H Bergman

Abstract

Bacillus species are spore-forming bacteria that are ubiquitous in the environment and display a range of virulent and avirulent phenotypes. This range is particularly evident in the Bacillus cereus sensu lato group; where closely related strains cause anthrax, food-borne illnesses, and pneumonia, but can also be non-pathogenic. Although much of this phenotypic range can be attributed to the presence or absence of a few key virulence factors, there are other virulence-associated loci that are conserved throughout the B. cereus group, and we hypothesized that these genes may be regulated differently in pathogenic and non-pathogenic strains. Here we report transcriptional profiles of three closely related but phenotypically unique members of the Bacillus cereus group--a pneumonia-causing B. cereus strain (G9241), an attenuated strain of B. anthracis (Sterne 34F(2)), and an avirulent B. cereus strain (10987)--during exponential growth in two distinct atmospheric environments: 14% CO(2)/bicarbonate and ambient air. We show that the disease-causing Bacillus strains undergo more distinctive transcriptional changes between the two environments, and that the expression of plasmid-encoded virulence genes was increased exclusively in the...Continue Reading

References

Aug 1, 1985·Infection and Immunity·B D GreenB E Ivins
Mar 1, 1982·The British Journal of Ophthalmology·P C HoW S Head
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·E MulliezP Reichard
Oct 1, 1994·Letters in Applied Microbiology·Les W BaillieR J Manchee
Nov 1, 1995·Research in Microbiology·J C SirardAgnès Fouet
Apr 19, 1996·The Journal of Biological Chemistry·S OllagnierP Reichard
Mar 22, 1996·The Journal of Biological Chemistry·X SunBritt-Marie Sjöberg
Feb 1, 1997·Journal of Clinical Microbiology·J M MillerRobbin S Weyant
Nov 26, 1998·International Journal of Systematic Bacteriology·S LechnerS Scherer
Dec 16, 1998·Journal of Bacteriology·L WinstedtC von Wachenfeldt
Jun 15, 1999·Trends in Microbiology·U B Sleytr, T J Beveridge
Sep 4, 1999·Journal of Applied Microbiology·Alex R Hoffmaster, Theresa M Koehler
Dec 3, 1999·Applied and Environmental Microbiology·B M PrüssS Scherer
Sep 12, 2000·FEMS Microbiology Letters·A M Gaviria RiveraFergus G Priest
Dec 9, 2000·International Journal of Medical Microbiology : IJMM·J C SirardMichèle E Mock
Oct 11, 2002·Journal of Bacteriology·Noel Baichoo, John D Helmann
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Apr 22, 2003·Infection and Immunity·Agathe BourgogneTheresa M Koehler
Jul 23, 2003·Environmental Microbiology·G B JensenJ Mahillon
Oct 1, 1957·Journal of Bacteriology·M STERNE, H PROOM
Oct 2, 2003·Genome Biology·Douglas A HosackRichard A Lempicki
Dec 18, 2003·Journal of Bacteriology·Hongbin LiuPhilip C Hanna
Jan 2, 2004·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler
Feb 6, 2004·Molecular Microbiology·Stephen CendrowskiPhilip C Hanna
May 20, 2004·International Journal of Medical Microbiology : IJMM·Tâm MignotAgnès Fouet
May 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alex R HoffmasterClaire M Fraser
Sep 11, 2004·International Journal of Food Microbiology·E L JääskeläinenM S Salkinoja-Salonen
Apr 6, 2005·FEMS Microbiology Reviews·David A RaskoJacques Ravel
Jul 21, 2005·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler
Dec 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Eduard TorrentsBritt-Marie Sjöberg
Jun 28, 2006·Current Microbiology·Ouassila OuhibPhilippe Schmitt
Jul 18, 2006·FEMS Microbiology Letters·Monika Ehling-SchulzBirgitta Svensson
Sep 7, 2006·Journal of Clinical Microbiology·David SuePatricia P Wilkins
Jan 12, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Joseph R Casey
May 2, 2007·Infection and Immunity·Nicholas H BergmanPhilip C Hanna
Jul 31, 2007·Future Microbiology·Karla D Passalacqua, Nicholas H Bergman
Dec 18, 2007·Applied and Environmental Microbiology·Mark L TamplinLynda C Kelley
Jan 15, 2008·Applied and Environmental Microbiology·K Eric WommackJacques Ravel
Mar 28, 2008·Molecular Microbiology·Justin W Kern, Olaf Schneewind
Jul 4, 2008·PloS One·Michael E ZwickTimothy D Read
Jul 31, 2008·PloS One·Michel GoharDidier Lereclus
Sep 13, 2008·Science·Michael D WilsonDuncan T Odom
Oct 28, 2008·Journal of Bacteriology·Jacques RavelDavid A Rasko
Nov 22, 2008·PLoS Pathogens·Adam C WilsonMarta Perego

Citations

Sep 21, 2013·Cellular and Molecular Life Sciences : CMLS·Eoin P CumminsCormac T Taylor
Oct 15, 2009·Future Microbiology·Theodor Chitlaru, Avigdor Shafferman
Mar 24, 2009·Journal of Bacteriology·Karla D PassalacquaNicholas H Bergman
Jan 9, 2013·Microbial Informatics and Experimentation·Clelia PeanoPaolo Landini
Aug 7, 2010·Microbiology·Inka SastallaStephen H Leppla
Dec 20, 2011·Gene·Ramakrishnan Sitaraman, Stephen H Leppla
Sep 25, 2010·Research in Microbiology·Agnès Fouet
Apr 2, 2015·PLoS Neglected Tropical Diseases·Christophe BrézillonPierre L Goossens
Apr 4, 2018·Infection and Immunity·Yuliya I SeldinaAlison D O'Brien
Oct 28, 2018·Applied and Environmental Microbiology·Barak DrorDani Eshel
Jan 1, 2014·International Scholarly Research Notices·Vicki Ann LunaDamian H Gilling
May 22, 2019·Microbiology Spectrum·Monika Ehling-SchulzTheresa M Koehler

Datasets Mentioned

BETA
GBAA2920-22
E-MEXP-2036

Methods Mentioned

BETA
transcriptional
chip
Electrophoresis
chips
PCR
phase contrast microscopy

Related Concepts

Related Feeds

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.