May 29, 2020

Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton

BMC Plant Biology
Kashif ShahzadChaozhu Xing

Abstract

Utilization of heterosis has greatly improved the productivity of many crops worldwide. Understanding the potential molecular mechanism about how hybridization produces superior yield in upland cotton is critical for efficient breeding programs. In this study, high, medium, and low hybrids varying in the level of yield heterosis were screened based on field experimentation of different years and locations. Phenotypically, high hybrid produced a mean of 14% more seed cotton yield than its better parent. Whole-genome RNA sequencing of these hybrids and their four inbred parents was performed using different tissues of the squaring stage. Comparative transcriptomic differences in each hybrid parent triad revealed a higher percentage of differentially expressed genes (DEGs) in each tissue. Expression level dominance analysis identified majority of hybrids DEGs were biased towards parent like expressions. An array of DEGs involved in ATP and protein binding, membrane, cell wall, mitochondrion, and protein phosphorylation had more functional annotations in hybrids. Sugar metabolic and plant hormone signal transduction pathways were most enriched in each hybrid. Further, these two pathways had most mapped DEGs on known seed cotton yie...Continue Reading

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Mentioned in this Paper

Gene Annotation
Comparative Genomic Analysis
Analysis
Breeding
Study
Gp 118 protein, Herpesvirus 3, Human
Gene Expression Profiling
Genes
Quantitative Reverse Transcriptase PCR
Transcriptome

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