Comparative Transcriptome Analysis Identifies Putative Genes Involved in Dioscin Biosynthesis in Dioscorea zingiberensis

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Jia LiYansheng Zhang

Abstract

Dioscorea zingiberensis is a perennial herb native to China. The rhizome of D. zingiberensis has long been used as a traditional Chinese medicine to treat rheumatic arthritis. Dioscin is the major bioactive ingredient conferring the medicinal property described in Chinese pharmacopoeia. Several previous studies have suggested cholesterol as the intermediate to the biosynthesis of dioscin, however, the biosynthetic steps to dioscin after cholesterol remain unknown. In this study, a comprehensive D. zingiberensis transcriptome derived from its leaf and rhizome was constructed. Based on the annotation using various public databases, all possible enzymes in the biosynthetic steps to cholesterol were identified. In the late steps beyond cholesterol, cholesterol undergoes site-specific oxidation by cytochrome P450s (CYPs) and glycosylation by UDP-glycosyltransferases (UGTs) to yield dioscin. From the D. zingiberensis transcriptome, a total of 485 unigenes were annotated as CYPs and 195 unigenes with a sequence length above 1000 bp were annotated as UGTs. Transcriptomic comparison revealed 165 CYP annotated unigenes correlating to dioscin biosynthesis in the plant. Further phylogenetic analysis suggested that among those CYP candidate...Continue Reading

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Citations

Mar 7, 2020·Journal of Asian Natural Products Research·Si-Yuan GuoJia Li
Sep 13, 2019·BioMed Research International·Longfei YangLufei Wang
May 18, 2021·Synthetic and Systems Biotechnology·Hui WangHuifeng Jiang
Nov 25, 2021·The Plant Journal : for Cell and Molecular Biology·Chen ZhouYansheng Zhang

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Datasets Mentioned

BETA
SRR6281651

Methods Mentioned

BETA
RNA-Seq
SGA
glycosylation

Software Mentioned

ClustalW
BLASTx
Trinity
MEGA
Cd
hit
BLASTP

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