Comparative Transcriptome Analysis of the Pinewood Nematode Bursaphelenchus xylophilus Reveals the Molecular Mechanism Underlying Its Defense Response to Host-Derived α-pinene

International Journal of Molecular Sciences
Yongxia LiXing-Yao Zhang

Abstract

Bursaphelenchus xylophilus is fatal to the pine trees around the world. The production of the pine tree secondary metabolite gradually increases in response to a B. xylophilus infestation, via a stress reaction mechanism(s). α-pinene is needed to combat the early stages of B. xylophilus infection and colonization, and to counter its pathogenesis. Therefore, research is needed to characterize the underlying molecular response(s) of B. xylophilus to resist α-pinene. We examined the effects of different concentrations of α-pinene on the mortality and reproduction rate of B. xylophilus in vitro. The molecular response by which B. xylophilus resists α-pinene was examined via comparative transcriptomics of the nematode. Notably, B. xylophilus genes involved in detoxification, transport, and receptor activities were differentially expressed in response to two different concentrations of α-pinene compared with control. Our results contribute to our understanding of the molecular mechanisms by which B. xylophilus responds to monoterpenes in general, and the pathogenesis of B. xylophilus.

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Citations

Feb 7, 2020·International Journal of Molecular Sciences·Fanli MengXingyao Zhang
Nov 11, 2020·Molecular Genetics and Genomics : MGG·Anna FilipiakMarek Tomalak
May 29, 2021·Frontiers in Plant Science·Hugo SilvaJoana M S Cardoso

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Datasets Mentioned

BETA
CADV01000001
CADV01010432

Methods Mentioned

BETA
reverse-transcription PCR
environmental stress
chips
PCR

Software Mentioned

Blast2GO
Origin
SPSS
Primer Premier
Tophat
WEGO
Trimmomatic
DESeq R package
HTSeq

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