Comparative Transcriptome Analysis Revealed Genes Regulated by Histone Acetylation and Genes Related to Sex Hormone Biosynthesis in Phytophthora infestans

Frontiers in Genetics
Xiao-Wen WangLi-Yun Guo

Abstract

Late blight caused by Phytophthora infestans, is one of the most devastating diseases of potato, and was responsible for the death of millions of people during the Irish Potato Famine in the nineteenth century. Phytophthora infestans is a heterothallic oomycete that typically requires two compatible types (mating types), A1 and A2, to complete sexual reproduction (i.e., oospore production). Oospores have critical effects on disease epidemiology because they serve as the primary inoculum in subsequent growing seasons. The sexual reproduction of Phytophthora species is regulated by α hormones. In previous studies, we proved that transformants in which selected histone deacetylase (HDAC) genes are silenced exhibit abnormal hormone production. In the current study, we compared the transcriptomes of HDAC-silenced and wild-type strains to explore the genes regulated by HDAC and the genes involved in sex hormone biosynthesis in Phytophthora species. A total of 14,423 transcripts of unigenes were identified in the wild-type strain, the HDAC family-silenced transformant (HDST), and the HDAC7-silenced transformant (H7ST). After comparing the intergroup gene expression levels, 1,612 unigenes were identified as differentially expressed amo...Continue Reading

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Citations

Apr 21, 2021·Environmental Microbiology Reports·Fernando Uriel Rojas-Rojas, Julio C Vega-Arreguín

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Methods Mentioned

BETA
histone acetylation
acetylation
RNA-seq
electrophoresis
PCR
Illumina
histone
Illumina sequencing

Software Mentioned

HTSeq
ABI 7500
KOBAS
DESeq R
BLAST
seq R package
TopHat2
GO

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