Comparative transcriptome analysis reveals novel roles of the Ras and cyclic AMP signaling pathways in environmental stress response and antifungal drug sensitivity in Cryptococcus neoformans.

Eukaryotic Cell
Shinae MaengYong-Sun Bahn

Abstract

The cyclic AMP (cAMP) pathway plays a central role in the growth, differentiation, and virulence of pathogenic fungi, including Cryptococcus neoformans. Three upstream signaling regulators of adenylyl cyclase (Cac1), Ras, Aca1, and Gpa1, have been demonstrated to control the cAMP pathway in C. neoformans, but their functional relationship remains elusive. We performed a genome-wide transcriptome analysis with a DNA microarray using the ras1Delta, gpa1Delta, cac1Delta, aca1Delta, and pka1Delta pka2Delta mutants. The aca1Delta, gpa1Delta, cac1Delta, and pka1Delta pka2Delta mutants displayed similar transcriptome patterns, whereas the ras1Delta mutant exhibited transcriptome patterns distinct from those of the wild type and the cAMP mutants. Interestingly, a number of environmental stress response genes are modulated differentially in the ras1Delta and cAMP mutants. In fact, the Ras signaling pathway was found to be involved in osmotic and genotoxic stress responses and the maintenance of cell wall integrity via the Cdc24-dependent signaling pathway. Notably, the Ras and cAMP mutants exhibited hypersensitivity to a polyene drug, amphotericin B, without showing effects on ergosterol biosynthesis, which suggested a novel method of a...Continue Reading

References

Aug 1, 1992·Molecular Microbiology·J C VarelaW H Mager
Mar 1, 1991·Molecular and Cellular Biology·J E GerstJ Field
Aug 1, 1990·Molecular & General Genetics : MGG·U M Praekelt, P A Meacock
Jan 1, 1988·Molecular and Cellular Biology·R B Wilson, K Tatchell
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P SassM Wigler
Aug 1, 1985·The Journal of Clinical Investigation·D L GrangerD T Durack
Nov 10, 1998·Fungal Genetics and Biology : FG & B·J A AlspaughJ Heitman
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·L S Robertson, G R Fink
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BuckL R Levin
Jul 17, 1999·Yeast·A Garay-Arroyo, A A Covarrubias
Sep 17, 1999·Journal of Clinical Microbiology·B A Arthington-SkaggsC J Morrison
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·K B LengelerJ Heitman
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew V Hubberstey, Emilio P Mottillo
Jan 30, 2003·Fungal Genetics and Biology : FG & B·Michael S WaughJ Andrew Alspaugh
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
Apr 19, 2003·FEMS Yeast Research·Filip RollandJohan M Thevelein
Oct 24, 2003·The Journal of Biological Chemistry·Ahmet KoçGary F Merrill
Jan 22, 2004·FEMS Yeast Research·Read Pukkila-Worley, J Andrew Alspaugh
Feb 25, 2005·Molecular Biology of the Cell·Yong-Sun BahnJoseph Heitman
Aug 10, 2005·Eukaryotic Cell·Weihua FanJoseph Heitman

❮ Previous
Next ❯

Citations

Oct 6, 2011·Journal of Proteome Research·Juliana CrestaniMarilene Henning Vainstein
Nov 15, 2011·Nature Reviews. Microbiology·Kathleen A McDonough, Ana Rodriguez
Jul 6, 2012·Clinical Microbiology Reviews·Teresa R O'Meara, J Andrew Alspaugh
Oct 9, 2012·Eukaryotic Cell·Dong-Hoon YangYong-Sun Bahn
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Rebecca S ShapiroLeah E Cowen
Oct 1, 2013·Eukaryotic Cell·Yong-Sun Bahn, Kwang-Woo Jung
Nov 12, 2010·Fungal Genetics and Biology : FG & B·Guilhem JanbonYong-Sun Bahn
Sep 12, 2013·Protein Science : a Publication of the Protein Society·Grainne O'KeeffeSean Doyle
Feb 6, 2014·Wiley Interdisciplinary Reviews. RNA·Amanda L M Bloom, John C Panepinto
Jun 27, 2015·Fungal Genetics and Biology : FG & B·Shannon K EsherJ Andrew Alspaugh
Sep 30, 2016·Nature Communications·Kyung-Tae LeeYong-Sun Bahn
May 24, 2012·Biotechnology & Genetic Engineering Reviews·Marjatta RaudaskoskiJ Stephen Horton
Apr 24, 2013·Mycobiology·Dong-Hoon YangYong-Sun Bahn
Jul 12, 2012·Mycobiology·James W KronstadJaehyuk Choi
May 13, 2014·Eukaryotic Cell·Diane O InglisJason E Stajich
Apr 15, 2017·Applied Microbiology and Biotechnology·Ningzi GuanLong Liu
Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Mélissa Caza, James W Kronstad
May 20, 2017·Wiley Interdisciplinary Reviews. RNA·Amanda L M BloomJohn C Panepinto
Mar 9, 2021·Genetics·Piotr R StempinskiLukasz Kozubowski
Apr 4, 2021·Journal of Fungi·Linda C Horianopoulos, James W Kronstad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.