Comparative transcriptome analysis to unveil genes affecting recombinant protein productivity in mammalian cells

Biotechnology and Bioengineering
Joon Chong YeeWei-Shou Hu

Abstract

Low temperature culture (33 degrees C) has been shown to enhance the specific productivity of recombinant antibodies in Chinese hamster ovary (CHO) cells but did not affect antibody productivity in hybridoma (MAK) cells. We probed the transcriptional response of both cells undergoing temperature shift using cDNA microarrays. Among the orthologous gene probes, common trends in the expression changes between CHO and MAK are not prominent. Instead, many transcriptional changes were specific to only one cell line. Notably, oxidative phosphorylation and ribosomal genes were downregulated in MAK but not in CHO. Conversely, several protein trafficking genes and cytoskeleton elements were upregulated in CHO but remained unchanged in MAK. Interestingly, at 33 degrees C, immunoglobulin heavy and light chain showed no significant changes in CHO, but the immunoglobulin light chain was downregulated in MAK. Overall, a clear distinction in the transcriptional response to low temperature was seen in the two cell lines. To further elucidate the set of genes responsible for increased antibody productivity, the expression data of low temperature cultures was compared to that of butyrate treatment which increased specific antibody productivity in...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 22, 1986·Journal of Immunological Methods·S ReuvenyL Miller
Jan 1, 1994·Cytotechnology·R WeidemannG Kretzmer
Jan 1, 1994·Annual Review of Cell Biology·E L Holzbaur, R B Vallee
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·C BucciM Zerial
Jun 11, 1993·Nucleic Acids Research·R Pellé, N B Murphy
Jun 1, 1997·The Biochemical Journal·K W Wirtz
Jun 20, 1997·The Journal of Biological Chemistry·J H Exton
Jan 1, 1997·Annual Review of Biochemistry·T Y ChangD Cheng
Jul 11, 1998·The Journal of Biological Chemistry·A Siddhanta, D Shields
Oct 10, 1998·Biochimica Et Biophysica Acta·D A Los, N Murata
Jun 8, 1999·Molecular Biology of the Cell·M SteegmaierR H Scheller
Jul 9, 1999·Biotechnology and Bioengineering·H KaufmannJ E Bailey
Nov 24, 1999·Nature Cell Biology·E NielsenM Zerial
Dec 22, 1999·The Journal of Biological Chemistry·H P ChoS D Clarke
Sep 5, 2001·The EMBO Journal·C RaiborgH Stenmark
Jan 10, 2003·Nucleic Acids Research·Maximilian DiehnAsh A Alizadeh
Oct 24, 2003·Biotechnology and Bioengineering·Simone M SchatzFriedrich Scheiflinger
Dec 23, 2003·Journal of Biotechnology·Rashmi KorkeWei-Shou Hu
Jul 1, 1991·Trends in Cell Biology·A ClevesV Bankaitis
Mar 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Francois BlondeauPeter S McPherson
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Fusako NagaiHiro-omi Tamura
Apr 6, 2004·Journal of Biotechnology·Mariela Bollati FogolínRicardo Kratje
Nov 3, 2004·Biochimica Et Biophysica Acta·Dmitry A Los, Norio Murata
Dec 14, 2004·The Journal of Biological Chemistry·Valérie BeaulieuSylvie Breton
Jun 7, 2005·Trends in Biochemical Sciences·Cesare MontecuccoSergio Pantano
Jul 9, 2005·Biotechnology and Bioengineering·Katie Fraass WlaschinWei-Shou Hu
Sep 13, 2005·The Journal of Biological Chemistry·Mark LaranceDavid E James

❮ Previous
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Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Jee Yon KimGyun Min Lee
Jul 24, 2013·Journal of Biotechnology·Franck C CourtesDong-Yup Lee
Nov 3, 2015·Biotechnology Advances·Simon FischerKerstin Otte
Feb 5, 2013·Metabolic Engineering·Jens NiklasElmar Heinzle
Mar 27, 2012·Journal of Biotechnology·Paula MeleadyMartin Clynes
Nov 15, 2011·Biotechnology Advances·Diane M WuestKelvin H Lee
Dec 1, 2010·Journal of Biotechnology·Colin ClarkeMartin Clynes
Mar 6, 2012·Biotechnology and Bioengineering·Paula MeleadyNicole Borth
Dec 20, 2011·Biotechnology Journal·Stefanie DietmairNicholas E Timmins
May 20, 2011·Biotechnology Progress·Yueming QianZheng Jian Li
Sep 25, 2009·Biotechnology and Bioengineering·Jochen SchaubTorsten W Schulz
Oct 11, 2013·Biotechnology and Bioengineering·Nandita VishwanathanWei-Shou Hu
Mar 26, 2013·Current Opinion in Biotechnology·Helene Faustrup KildegaardMichael J Betenbaugh
Jul 12, 2014·Current Opinion in Biotechnology·Nandita VishwanathanWei-Shou Hu
Jul 13, 2014·Cancer Letters·Lufang ZhouXiaoguang Margaret Liu
Jan 7, 2015·Journal of Industrial Microbiology & Biotechnology·Tobias KleinElmar Heinzle
Apr 2, 2017·Journal of Biotechnology·B MüllerR Hoffrogge
Jan 10, 2018·Biotechnology Journal·Camila A OrellanaLars K Nielsen
Jan 14, 2020·Biotechnology and Bioengineering·Matthew N HenryVerónica S Martínez
Sep 1, 2020·Biotechnology and Bioengineering·Camila A OrellanaEsteban Marcellin

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