Comparative transcriptome and metabolome provides new insights into the regulatory mechanisms of accelerated senescence in litchi fruit after cold storage

Scientific Reports
Ze YunYueming Jiang

Abstract

Litchi is a non-climacteric subtropical fruit of high commercial value. The shelf life of litchi fruit under ambient conditions (AC) is approximately 4-6 days. Post-harvest cold storage prolongs the life of litchi fruit for up to 30 days with few changes in pericarp browning and total soluble solids. However, the shelf life of litchi fruits at ambient temperatures after pre-cold storage (PCS) is only 1-2 days. To better understand the mechanisms involved in the rapid fruit senescence induced by pre-cold storage, a transcriptome of litchi pericarp was constructed to assemble the reference genes, followed by comparative transcriptomic and metabolomic analyses. Results suggested that the senescence of harvested litchi fruit was likely to be an oxidative process initiated by ABA, including oxidation of lipids, polyphenols and anthocyanins. After cold storage, PCS fruit exhibited energy deficiency, and respiratory burst was elicited through aerobic and anaerobic respiration, which was regulated specifically by an up-regulated calcium signal, G-protein-coupled receptor signalling pathway and small GTPase-mediated signal transduction. The respiratory burst was largely associated with increased production of reactive oxygen species, up...Continue Reading

References

Aug 12, 2000·The Journal of Biological Chemistry·R ShirakawaT Kita
May 17, 2003·Journal of Plant Physiology·Maria Rosa AbenavoliAmodio Fuggi
May 8, 2004·Trends in Plant Science·Jozef SamajDiedrik Menzel
Jun 9, 2005·Current Opinion in Plant Biology·Miguel Angel Torres, Jeffery L Dangl
Dec 13, 2006·Trends in Plant Science·Lucille PourcelIsabelle Debeaujon
Dec 21, 2006·Annual Review of Plant Biology·Pyung Ok LimHong Gil Nam
Jan 12, 2007·Molecular BioSystems·Amandine Chefson, Karine Auclair
Dec 14, 2007·Nature·Claus OlesenPoul Nissen
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Nov 9, 2011·Annual Review of Genetics·Harry J Klee, James J Giovannoni

❮ Previous
Next ❯

Citations

Mar 24, 2018·International Journal of Molecular Sciences·Yuanzheng YueHuirong Hu
May 3, 2018·Annals of Botany·Bertrand BeauvoitYves Gibon
Nov 13, 2018·Journal of the Science of Food and Agriculture·Qixian WuYueming Jiang
May 6, 2019·Metabolomics : Official Journal of the Metabolomic Society·Anjaritha A R ParijadiEiichiro Fukusaki
Mar 3, 2021·Food Research International·Xi ChenYueming Jiang

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRA247016

Methods Mentioned

BETA
GTPases
GTPase
reverse-transcription PCR
RNA Assay
Assay
PCR
DNA assay

Software Mentioned

blastx
BiNGO
Blastall
Blast2GO
SOAP
Perl scripts
Primer Express
Trinity
ESTScan

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.