Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis

Scientific Reports
Manish TiwariShivendra V Sahi

Abstract

A large number of plants have been tested and exploited in search of a green chemistry approach for the fabrication of gold or other precious metal nanomaterials. Despite the potential of plant based methods, very little is known about the underlying biochemical reactions and genes involved in the biotransformation mechanism of AuCl4 into gold nanoparticles (AuNPs). In this research, we thus focused on studying the effect of Au on growth and nanoparticles formation by analyses of transcriptome, proteome and ionome shift in Arabidopsis. Au exposure favored the growth of Arabidopsis seedling and induced formation of nanoparticles in root and shoot, as indicated by optical and hyperspectral imaging. Root transcriptome analysis demonstrated the differential expression of the members of WRKY, MYB and BHLH gene families, which are involved in the Fe and other essential metals homeostasis. The proteome analysis revealed that Glutathione S-transferases were induced in the shoot and suggested its potential role in the biosynthesis AuNPs. This study also demonstrated the role of plant hormone auxin in determining the Au induced root system architecture. This is the first study using an integrated approach to understand the in planta biot...Continue Reading

References

Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 22, 2004·The Plant Cell·Dawar HussainChristopher S Cobbett
Jun 24, 2006·The Plant Journal : for Cell and Molecular Biology·Jae-Heung KoKyung-Hwan Han
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Aug 23, 2007·Environmental Science & Technology·Nilesh C SharmaTarasankar Pal
Nov 28, 2008·Plant Physiology·Mélanie MorelPierre Richaud
Mar 3, 2009·Phytochemistry·Yoshitaka GogamiTadao Oikawa
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Reetika RawatStacey L Harmer
Oct 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Frank ReithJoël Brugger
Sep 3, 2010·The Plant Journal : for Cell and Molecular Biology·Hyung-Sae KimSang-Bong Choi
May 2, 2012·Environmental Science & Technology·Appala Raju BadireddyMark R Wiesner
Jun 26, 2012·Plant Physiology·Alberto P MachoCyril Zipfel
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alvina Grace LaiPaul P Dijkwel
Oct 23, 2012·Nature·Henning TidowPoul Nissen
Oct 26, 2012·Journal of Experimental Botany·Kerstin MüllerAllison R Kermode
Feb 5, 2013·Nature Chemical Biology·Chad W JohnstonNathan A Magarvey
May 2, 2013·Nucleic Acids Research·Xin YiZhen Su
Oct 15, 2013·The Plant Journal : for Cell and Molecular Biology·Zhong Jie DingShao Jian Zheng
Jan 1, 2008·Nanotechnology, Science and Applications·Po C ChenAdegboyega K Oyelere
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Takao ArayaHideki Takahashi

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Citations

Jun 5, 2019·Environmental Science and Pollution Research International·Petra Peharec ŠtefanićBiljana Balen
Aug 21, 2019·Scientific Reports·Sinilal BhaskaranShivendra V Sahi
Jun 13, 2017·Annual Review of Analytical Chemistry·Seon-Yeong KwakMichael S Strano
Jan 2, 2020·Journal of Environmental Sciences (China)·Supriyanka RanaLakhveer Singh
Feb 12, 2021·The Science of the Total Environment·Rawil FakhrullinGölnur Fakhrullina

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Methods Mentioned

BETA
RSA
environmental stresses
Protein
electrophoresis

Software Mentioned

Image
Partek Genomics Suite
GSEA
Plant GeneSet Enrichment Analysis Tool kit
ImageMaster 2D Platinum
CytoViva
Spectral Angle Mapper ( SAM )
MASCOT
AGCC
Plant Gene Set Enrichment Analysis ( Plant GSEA )

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